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Bioactivity assessment, micropollutant and nutrient removal ability of Tetradesmus obliquus cultivated outdoors in centrate from urban wastewater

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Abstract

Nutrient and micropollutant removal, and bioactivity were studied in cultures of the green microalga Tetradesmus obliquus MACC-677 grown in centrate from municipal wastewater (WW). Two outdoor units, a thin-layer cascade (TLC) and a thin-layer raceway pond (TL-RWP), were tested for microalgal culturing in batch and semi-continuous regimes where their photosynthetic performance was monitored. The results revealed that the T. obliquus cultures grew well, showing a high specific growth rate µ of 0.31 day−1 and 0.25 day−1 when grown in WW in TLC and TL-RWP, respectively. The cultivation trials showed high nutrient removal efficiency for ammonium nitrogen (98.5%) as well as orthophosphate (89%), the most abundant forms of N and P occurring in municipal WW. The removal of selected pharmaceuticals and endocrine disruptors (e.g., ibuprofen, amitriptyline, bisphenol A, etc.) was also assessed. Ibuprofen was the most abundant micropollutant detected in the centrate, with concentrations up to 5000 ng L−1 and fast removal during the cultivation. The biomass produced in the centrate revealed antimicrobial activity against plant pathogens, including fungi, oomycota, and bacteria. These findings have shown that the culturing of T. obliquus can be considered a suitable way to contribute to a circular economy, to remove nutrients and micropollutants from municipal WW from which biomass extracts can be further used for plant protection in agriculture.

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Data availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors thank Ms. Soňa Pekařová, Mr. Michal Bureš, Mr. Petr Novotný and Mr. Daniyar Malikuly Kozhan for technical assistance during cultivation and Mr. Miroslav Kajan for consultation on wastewater use.

Funding

This work was supported by the EU program Horizon 2020 [project SABANA, grant No.727874] and partly supported by the Program INTERREG V-A Austria – Czech Republic 2014 – 2020 [project Plastocyan ATCZ260], and the ESF project International mobility of researchers—MSCA-IF III (Institute of Microbiology of the CAS, v.v.i.), [reg. nr. CZ.02.2.69/0.0/0.0/19_074/0014484].

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Authors and Affiliations

  1. Centre Algatech, Laboratory of Algal Biotechnology, Institute of Microbiology of the Czech Academy of Sciences, Novohradská 237, 379 01, Třeboň, Czech Republic

    Tomáš Grivalský, Karolína Ranglová, Gergely Ernö Lakatos, João Artur Câmara Manoel & Jiří Masojídek

  2. Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic

    João Artur Câmara Manoel & Jiří Masojídek

  3. Laboratory of Environmental Biotechnology, Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic

    Tereza Černá

  4. Faculty of Science, Charles Univerzity, Prague, Czech Republic

    Tereza Černá

  5. Department of Chemical Engineering, University of Almería, Almería, Spain

    Marta Barceló-Villalobos

  6. Department of Biology and Geology, University of Almería, Almería, Spain

    Francisca Suárez Estrella

  7. Faculty of Agricultural and Food Science, Szechényi István University, Mosonmagyaróvár, Hungary

    Vince Ördög

  8. Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal, Pietermaritzburg, South Africa

    Vince Ördög

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  1. Tomáš Grivalský
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Contributions

Tomáš Grivalský, Jiří Masojídek, Vince Ördög conceived research. Tomáš Grivalský, Karolína Ranglová, Gergely Ernö Lakatos, João Artur Câmara Manoel, Tereza Černá, Marta Barceló-Villalobos, Francisca Suárez Estrella, analysed data. Tomáš Grivalský prepared the text of the manuscript. Tomáš Grivalský, Karolína Ranglová, Gergely Ernö Lakatos and Tereza Černá prepared the figures of the manuscript. Jiří Masojídek, Vince Ördög revised and edited the manuscript. Tomáš Grivalský finalized the manuscript. All authors read and approved the manuscript.

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Grivalský, T., Ranglová, K., Lakatos, G.E. et al. Bioactivity assessment, micropollutant and nutrient removal ability of Tetradesmus obliquus cultivated outdoors in centrate from urban wastewater. J Appl Phycol 34, 2955–2970 (2022). https://doi.org/10.1007/s10811-022-02828-6

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