Skip to main content
SpringerLink
Log in

Seasonal dynamics of aerobic anoxygenic phototrophs in freshwater lake Vlkov

  • Original Article
  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

Aerobic anoxygenic phototrophic (AAP) bacteria are a common component of freshwater microbial communities. They harvest light energy using bacteriochlorophyll a-containing reaction centers to supplement their predominantly heterotrophic metabolism. We used epifluorescence microscopy, HPLC, and infrared fluorometry to examine the dynamics of AAP bacteria in the mesotrophic lake Vlkov during the seasonal cycle. The mortality of AAP bacteria was estimated from diel changes of bacteriochlorophyll a fluorescence. The AAP abundance correlated with water temperature and DOC concentration. Its maximum was registered during late summer, when AAP bacteria made up 20% of total bacteria. The novel element of this study is the seasonal measurements of AAP mortality rates. The rates ranged between 1.15 and 4.56 per day with the maxima registered in early summer coinciding with the peak of primary production, which documents that AAP bacteria are a highly active component of freshwater microbial loop.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Anderson MJ, Legendre P (1999) An empirical comparison of permutation methods for tests of partial regression coefficients in a linear model. J Stat Comput Simul 62:271–303

    Article  Google Scholar 

  • Caliz J, Casamayor EO (2014) Environmental controls and composition of anoxygenic photoheterotrophs in ultraoligotrophic high-altitude lakes (Central Pyrenees). Environ Microbiol Rep 6:145–151

    Article  CAS  Google Scholar 

  • Cepáková Z, Hrouzek P, Žišková E, Nuyanzina-Boldareva E, Šorf M, Kozlíková-Zapomělová E, Salka I, Grossart HP, Koblížek M (2016) High turnover rates of aerobic anoxygenic phototrophs in European freshwater lakes. Environ Microbiol 18:5063–5071

    Article  Google Scholar 

  • Čuperová Z, Holzer E, Salka I, Sommaruga R, Koblížek M (2013) Temporal changes and altitudinal distribution of aerobic anoxygenic phototrophs in mountain lakes. Appl Environ Microbiol 79:6439–6446

    Article  Google Scholar 

  • Fauteux L, Cottrell MT, Kirchman DL, Borrego CM, Garcia-Chavez MC, del Giorgio PA (2015) Patterns in abundance, cell size and pigment content of aerobic anoxygenic phototrophic bacteria along environmental gradients in northern lakes. PLoS One 10:e0124035

    Article  Google Scholar 

  • Ferrera I, Gasol JM, Sebastián M, Hojerová E, Koblížek M (2011) Comparison of growth rates of aerobic anoxygenic phototrophic bacteria and other bacterioplankton in coastal Mediterranean Waters. Appl Environ Microbiol 77:7451–7458

    Article  CAS  Google Scholar 

  • Ferrera I, Sanchez O, Kolářová E, Koblížek M, Gasol JM (2017a) Light enhances the growth rates of natural populations of aerobic anoxygenic phototrophic bacteria. ISME J 11:2391–2393

    Article  CAS  Google Scholar 

  • Ferrera I, Sarmento H, Priscu JC, Chiuchiolo A, González JM, Grossart H-P (2017b) Diversity and distribution of freshwater aerobic anoxygenic phototrophic bacteria across a wide latitudinal gradient. Front Microbiol 8:175. https://doi.org/10.3389/fmicb.2017.00175

    Article  PubMed  PubMed Central  Google Scholar 

  • Garcia-Chaves MC, Cottrell MT, Kirchman DL, Derry AM, Bogard MJ, del Giorgio PA (2015) Major contribution of both zooplankton and protists to the top-down regulation of freshwater aerobic anoxygenic phototrophic bacteria. Aquat Microb Ecol 76:71–83

    Article  Google Scholar 

  • Garcia-Chaves MC, Cottrell MT, Kirchman DL, Ruiz-González C, del Giorgio PA (2016) Single-cell activity of freshwater aerobic anoxygenic phototrophic bacteria and their contribution to biomass production. ISME J 10:1579–1588

    Article  CAS  Google Scholar 

  • Hauruseu D, Koblížek M (2012) The influence of light on carbon utilization in aerobic anoxygenic phototrophs. Appl Environ Microbiol 78:7414–7419

    Article  CAS  Google Scholar 

  • Huang Y, Zeng Y, Lu H, Feng H, Zeng Y, Koblížek M (2016) Novel acsF gene primers revealed a diverse phototrophic bacterial population, including Gemmatimonadetes, in Lake Taihu (China). Appl Environ Microbiol 82:5587–5594

    Article  CAS  Google Scholar 

  • Koblížek M (2015) Ecology of aerobic anoxygenic phototrophs in aquatic environments. FEMS Microbiol Rev 39:854–870

    Article  Google Scholar 

  • Koblížek M, Mlčoušková J, Kolber Z, Kopecký J (2010) On the photosynthetic properties of marine bacterium COL2P belonging to Roseobacter clade. Arch Microbiol 192:41–49

    Article  Google Scholar 

  • Koblížek M, Ston-Egiert J, Sagan S, Kolber ZS (2005) Diel changes in bacteriochlorophyll a concentration suggest rapid bacterioplankton cycling in the Baltic Sea. FEMS Microb Ecol 51:353–361

    Article  Google Scholar 

  • Legendre P, Anderson MJ (1999) Distance-based redundancy analysis: testing multispecies responses in multifactorial ecological experiments. Ecol Monogr 69:1–24

    Article  Google Scholar 

  • Lew S, Koblížek M, Lew M, Medová H, Glińska-Lewczuk K, Owsianny PM (2015) Seasonal changes of microbial communities in two shallow peat-bog lakes. Folia Microbiol 60:165–175

    Article  CAS  Google Scholar 

  • Liu R, Zhang Y, Jiao N (2010) Diel changes in frequency of dividing cells and abundance of aerobic anoxygenic phototrophic bacteria in coral reef system of the South China Sea. Aquat Microb Ecol 58:303–310

    Article  Google Scholar 

  • Mašín M, Nedoma J, Pechar L, Koblížek M (2008) Distribution of aerobic anoxygenic phototrophs in temperate freshwater systems. Environ Microbiol 10:1988–1996

    Article  Google Scholar 

  • Mašín M, Čuperová Z, Hojerová E, Salka I, Grossart H-P, Koblížek M (2012) Distribution of aerobic anoxygenic phototrophic bacteria in glacial lakes of Northern Europe. Aquat Microb Ecol 66:77–86

    Article  Google Scholar 

  • Piwosz K, Kaftan D, Dean J, Šetlík J, Koblížek M (2018) Nonlinear effect of irradiance on photoheterotrophic activity and growth of the aerobic anoxygenic phototrophic bacterium Dinoroseobacter shibae. Environ Microbiol 20:724–733

    Article  CAS  Google Scholar 

  • Ruiz-González C, Proia L, Ferrera I, Gasol JM, Sabater S (2013) Effects of large river dam regulation on bacterioplankton community structure. FEMS Microbiol Ecol 84:316–331

    Article  Google Scholar 

  • Salka I, Čuperová Z, Mašín M, Koblížek M, Grossart H-P (2011) Rhodoferax-related pufM gene cluster dominates the aerobic anoxygenic phototrophic communities in German freshwater lakes. Environ Microbiol 13:2865–2875

    Article  CAS  Google Scholar 

  • Sato-Takabe Y, Hamasaki K, Suzuki S (2018) High temperature accelerates growth of aerobic anoxygenic phototrophic bacteria in seawater. MicrobiologyOpen 8:e00710. https://doi.org/10.1002/mbo3.710

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Shi L, Cai Y, Chen Z, Zhou Y, Li P, Kong F (2010) Diversity and abundance of aerobic anoxygenic phototrophic bacteria in two cyanobacterial bloom-forming lakes in China. Ann Limnol Int J Limnol 46:233–239

    Article  Google Scholar 

  • Šimek K, Horňák K, Jezbera J, Mašín M, Nedoma J, Gasol JM, Schauer M (2005) Influence of top-down and bottom-up manipulations on the R-BT065 subcluster of beta-proteobacteria, an abundant group in bacterioplankton of a freshwater reservoir. Appl Environ Microbiol 71:2381–2390

    Article  Google Scholar 

  • Šimek K, Horňák K, Jezbera J, Nedoma J, Vrba J, Straškrábová et al (2006) Maximum growth rates and possible life strategies of different bacterioplankton groups in relation to phosphorus availability in a freshwater reservoir. Environ Microbiol 8:1613–1624

    Article  Google Scholar 

  • Waidner LA, Kirchman DL (2005) Aerobic anoxygenic photosynthesis genes and operons in uncultured bacteria in the Delaware River. Environ Microbiol 7:1896–1908

    Article  CAS  Google Scholar 

  • Yurkov VV, Csotonyi JT (2009) New light on aerobic anoxygenic phototrophs. In: Hunter CN, Daldal F, Thurnauer MC, Beaty JT (eds) The purple phototrophic bacteria, advances in photosynthesis and respiration, vol 28. Springer Verlag, Dordrecht, pp 31–55

    Chapter  Google Scholar 

  • Yurkov VV, van Gemerden H (1993) Impact of light/dark regimen on growth rate, biomass formation and bacteriochlorophyll synthesis in Erythromicrobium hydrolyticum. Arch Microbiol 159:84–89

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors thank J. Dean BSc. for his help during the sampling and for the language corrections, and Eva Žišková for her help with pigment analyses.

Funding

This research was supported by the MŠMT project Algatech plus (LO1416).

Author information

Authors and Affiliations

  1. Center Algatech, Institute of Microbiology CAS, 379 81, Třeboň, Czech Republic

    Eva Kolářová, Hana Medová, Kasia Piwosz & Michal Koblížek

Authors
  1. Eva Kolářová
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hana Medová
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kasia Piwosz
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Michal Koblížek
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Michal Koblížek.

Additional information

Dedicated to the memory of Prof. Ivan Šetlík.

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kolářová, E., Medová, H., Piwosz, K. et al. Seasonal dynamics of aerobic anoxygenic phototrophs in freshwater lake Vlkov. Folia Microbiol 64, 705–710 (2019). https://doi.org/10.1007/s12223-019-00735-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12223-019-00735-x

Search

Navigation