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Antibacterial Silver-Conjugated Magnetic Nanoparticles: Design, Synthesis and Bactericidal Effect

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Abstract

Purpose

The aim was to design and thoroughly characterize monodisperse Fe3O4@SiO2-Ag nanoparticles with strong antibacterial properties, which makes them a candidate for targeting bacterial infections.

Methods

The monodisperse Fe3O4 nanoparticles were prepared by oleic acid-stabilized thermal decomposition of Fe(III) oleate; the particles were coated with silica shell using a water-in-oil reverse microemulsion, involving hydrolysis and condensation of tetramethyl orthosilicate. Resulting Fe3O4@SiO2 particles were modified by (3-mercaptopropyl)trimethoxysilane to introduce 1.1 mmol SH/g. Finally, the Fe3O4@SiO2-SH nanoparticles were decorated with silver nanoclusters formed by reduction of silver nitrate with NaBH4. The particles were analyzed by FTIR, X-ray photoelectron and atomic absorption spectroscopy, dynamic light scattering and vibrating sample magnetometry. The antibacterial activity of the Fe3O4@SiO2 and Fe3O4@SiO2-Ag nanoparticles was tested against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli bacteria cultivated on Luria agar plates or in Luria broth.

Results

The superparamagnetic Fe3O4@SiO2-Ag nanoparticles (21 nm in diameter; saturation magnetization 26 A∙m2/kg) were successfully obtained and characterized. Inhibitory and toxic effects against bacteria were documented by incubation of the Fe3O4@SiO2-Ag nanoparticles with Staphylococcus aureus and Escherichia coli.

Conclusions

The combination of magnetic properties together with bactericidal effects is suitable for the disinfection of medical instruments, water purification, food packaging, etc.

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Abbreviations

AAS:

Atomic absorption spectrometer

Amp:

Ampicillin

CFU:

Colony forming units

Ð :

Dispersity

D h :

Hydrodynamic diameter

DLS:

Dynamic light scattering

D n :

Number-average diameter

DT:

1-Dodecanethiol

D w :

Weight-average diameter

E. coli :

Escherichia coli

Igepal CO-520:

Polyoxyethylene(5) nonylphenylether

IS:

Icosane

LA:

Luria agar plates

LB:

Luria broth

MPTMS:

(3-Mercaptopropyl)trimethoxysilane

NT:

No treatment controls

OA:

Oleic acid

OD:

Octadec-1-ene

PBS:

Phosphate buffered saline

PI :

Polydispersity index

S. aureus :

Staphylococcus aureus

SAM:

Self-assembled monolayer

SBH:

Sodium borohydride

TEM:

Transmission electron microscope

TMOS:

Tetramethyl orthosilicate

XPS:

X-ray photoelectron spectroscopy

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Authors and Affiliations

  1. Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského nám. 2, 162 06, Prague 6, Czech Republic

    Anastasiia B. Shatan, Kristýna Venclíková, Beata A. Zasońska, Vitalii Patsula, Ognen Pop-Georgievski & Daniel Horák

  2. Institute of Geophysics, Czech Academy of Sciences, Boční II/1401, 141 31, Prague 4, Czech Republic

    Eduard Petrovský

Authors
  1. Anastasiia B. Shatan
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  2. Kristýna Venclíková
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Correspondence to Daniel Horák.

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Shatan, A.B., Venclíková, K., Zasońska, B.A. et al. Antibacterial Silver-Conjugated Magnetic Nanoparticles: Design, Synthesis and Bactericidal Effect. Pharm Res 36, 147 (2019). https://doi.org/10.1007/s11095-019-2680-x

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  • DOI: https://doi.org/10.1007/s11095-019-2680-x

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