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First ultrastructural and cytochemical data on the spermatozoon and its differentiation in progenetic and adult Archigetes sieboldi Leuckart, 1878 (Cestoda, Caryophyllidea, Caryophyllaeidae)

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Abstract

Spermiogenesis in progenetic and adult stages of Archigetes sieboldi Leuckart, 1878, a tapeworm parasitic in oligochaetes and fish respectively, has been examined using transmission electron microscopy and cytochemical staining for glycogen. General pattern of spermiogenesis is essentially like that of other caryophyllideans, i.e., apical dense material in the zone of differentiation in the early stages of spermiogenesis, rotation of free flagellum and a flagellar bud, and proximo-distal fusion. Interestingly, rotation of a free flagellum and flagellar bud to the median cytoplasmic process (MCP) has been observed unconventionally at > 90° only in progenetic stages. Typical striated roots associated with the centrioles occur rarely in A. sieboldi, and only in form of faint structures in advanced stages of spermiogenesis. In contrast to most caryophyllideans studied to date, penetration of the nucleus into the spermatid body has started before the fusion of the free flagellum with the MCP. This feature has been reported rarely but exclusively in the family Caryophyllaeidae. The unipartite mature spermatozoon of A. sieboldi is composed of one axoneme of the 9 + ‘1’ trepaxonematan pattern with its centriole, parallel nucleus, and parallel cortical microtubules which are situated in a moderately electron-dense cytoplasm with glycogen particles. An unusual arrangement of cortical microtubules in the two parallel rows in region I of the spermatozoon is described here for the first time in the Caryophyllidea. Ultrastructural data on spermiogenesis and the spermatozoon in A. sieboldi from tubuficids and carp are compared and discussed with those in other caryophyllideans and/or Neodermata.

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Acknowledgements

We would like to thank our colleague Mikuláš Oros (Slovakia) for providing invaluable help with collecting parasites. This study was supported by the Grant Agency of the Slovak Republic VEGA (project no. 2/0104/16 to MB). We acknowledge the core facility of the Institute of Parasitology, BC ASCR in České Budějovice, Czech Republic, supported by the MEYS CR (LM2015062 Czech-BioImaging). This research was undertaken within the framework of a joint research project nos. AV ČR-16-08 and SAV-18-21 supported by a bilateral agreement on scientific exchange and cooperation signed by the Czech and Slovak Academies of Sciences.

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  1. Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 040 01, Košice, Slovak Republic

    Magdaléna Bruňanská & Martina Matoušková

  2. Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, České Budějovice, Czech Republic

    Jana Nebesářová

  3. Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic

    Jana Nebesářová

  4. Department of Biological Sciences, University at Albany, State University of New York, Albany, NY, USA

    John S. Mackiewicz

  5. I. D. Papanin Institute for Biology of Inland Waters, Russian Academy of Sciences, Borok, Yaroslavl Province, Russia

    Larisa G. Poddubnaya

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  1. Magdaléna Bruňanská
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Correspondence to Magdaléna Bruňanská.

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Bruňanská, M., Matoušková, M., Nebesářová, J. et al. First ultrastructural and cytochemical data on the spermatozoon and its differentiation in progenetic and adult Archigetes sieboldi Leuckart, 1878 (Cestoda, Caryophyllidea, Caryophyllaeidae). Parasitol Res 118, 1205–1214 (2019). https://doi.org/10.1007/s00436-019-06276-z

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