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Aliphatic Hydrocarbon Enhances Phenanthrene Degradation by Autochthonous Prokaryotic Communities from a Pristine Seawater

  • Environmental Microbiology
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Abstract

The microbial diversity and functioning around oceanic islands is poorly described, despite its importance for ecosystem homeostasis. Here, we aimed to verify the occurrence of microbe-driven phenanthrene co-oxidation in the seawater surrounding the Trindade Island (Brazil). We also used Next-Generation Sequencing to evaluate the effects of aliphatic and polycyclic aromatic hydrocarbons (PAHs) on these microbial community assemblies. Microcosms containing seawater from the island enriched with either labelled (9-14C) or non-labelled phenanthrene together with hexadecane, weathered oil, fluoranthene or pyrene, and combinations of these compounds were incubated. Biodegradation of phenanthrene-9-14C was negatively affected in the presence of weathered oil and PAHs but increased in the presence of hexadecane. PAH contamination caused shifts in the seawater microbial community—from a highly diverse one dominated by Alphaproteobacteria to less diverse communities dominated by Gammaproteobacteria. Furthermore, the combination of PAHs exerted a compounded negative influence on the microbial community, reducing its diversity and thus functional capacity of the ecosystem. These results advance our understanding of bacterial community dynamics in response to contrasting qualities of hydrocarbon contamination. This understanding is fundamental in the application and monitoring of bioremediation strategies if accidents involving oil spillages occur near Trindade Island and similar ecosystems.

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Acknowledgments

We would like to thank the Brazilian Navy, Captain Rodrigo Otoch Chaves and Captain Sidnei da Costa Abrantes for the logistic support while collecting samples. This work was supported by the National Council for Scientific and Technological Development (Conselho Nacional de Desenvolvimento Científico e Tecnológico/CNPq) which provided all approvals and permits (project grant number 405544/2012-0 and authorisation access to genetic resources process number 010645/2013-6), FAPEMIG and CAPES. VSP receives fellowship from FAPESP (Process 2016/02219-8). This work was supported by the Brazilian Microbiome Project (http://www.brmicrobiome.org) and the National Institute of Science and Technology: Microbiome (http://www.inct-microbiome.org).

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Authors and Affiliations

  1. Laboratório de Biotecnologia e Biodiversidade para o Meio Ambiente, Departamento de Microbiologia, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil

    Edmo Montes Rodrigues & Marcos Rogério Tótola

  2. Laboratory of Environmental Microbiology, Institute of Microbiology of the Czech Institute of Sciences (CAS), Prague, Czech Republic

    Daniel Kumazawa Morais

  3. Soil Microbiology Laboratory, Department of Soil Science, “Luiz de Queiroz” College of Agriculture, ESALQ/USP, Piracicaba, São Paulo, Brazil

    Victor Satler Pylro

  4. Department of Sustainable Soils and Grassland Systems, Rothamsted Research, Harpenden, Hertfordshire, UK

    Marc Redmile-Gordon

  5. Laboratório de Biofísica Ambiental, Departamento de Biologia, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil

    Juraci Alves de Oliveira

  6. Centro para Pesquisa Interdisciplinar em Biotecnologia, CIP-Biotec, Universidade Federal do Pampa, São Gabriel, Rio Grande do Sul, Brazil

    Luiz Fernando Wurdig Roesch

  7. Laboratório de Ecologia e Biologia Molecular de Microrganismos, Departamento de Biologia, Universidade Federal de Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil

    Dionéia Evangelista Cesar

Authors
  1. Edmo Montes Rodrigues
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  2. Daniel Kumazawa Morais
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  3. Victor Satler Pylro
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  4. Marc Redmile-Gordon
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  5. Juraci Alves de Oliveira
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  6. Luiz Fernando Wurdig Roesch
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  7. Dionéia Evangelista Cesar
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  8. Marcos Rogério Tótola
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Corresponding author

Correspondence to Edmo Montes Rodrigues.

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Rodrigues, E.M., Morais, D.K., Pylro, V.S. et al. Aliphatic Hydrocarbon Enhances Phenanthrene Degradation by Autochthonous Prokaryotic Communities from a Pristine Seawater. Microb Ecol 75, 688–700 (2018). https://doi.org/10.1007/s00248-017-1078-8

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  • DOI: https://doi.org/10.1007/s00248-017-1078-8

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