Abstract
Polymethylmethacrylate (PMMA) bone cement mixed with antibiotics is used in orthopedic surgery to cope with implant-related infections which are typically associated with the formation of bacterial biofilms. Taking into account the growing bacterial resistance to current antibiotics, we examined here the efficacy of a selected antimicrobial peptide (AMP) mixed into the bone cement to inhibit bacterial adhesion and the consequent biofilm formation on its surface. In particular, we followed the formation of bacterial biofilms of methicillin-resistant Staphylococcus aureus (MRSA) on implants made from PMMA bone cement loaded with AMP composed of 12 amino acid residues. This was evaluated by CFU counting of bacteria released by sonication from the biofilms formed on their surfaces after these implants were retrieved from the infected murine femoral canals. The AMP loaded in these model implants prevented adhesion of MRSA and the subsequent formation of MRSA biofilm on the surfaces of more than 80% of these implants, whereas biofilms did form on control implants made from the plain cement. The results of our experiments performed in the murine femoral canal indicate the potential for this murine osteomyelitis model to mimic actual operations in orthopedics.
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Acknowledgements
We thank Dr. Radek Šerý from the National Institute of Public Health for his help with the experiments on laboratory animals. We also thank Tamara Jenkins for assistance with the English.
Funding
This work was supported by the Czech Health Research Council (AZV ČR, grant number 16-27726A), by research project RVO 61388963 of the Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, and by internal grants numbers 9777 (Advanced therapies) and 6010 of the Motol University Hospital.
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All applicable international, national, and institutional guidelines for the care and use of animals were followed. The experiments with animals were carried out in the research and breeding facility of the National Institute of Public Health in Prague, under conditions that comply with Regulation No. 419/2012 of the Ministry of Health as last amended. All procedures performed in studies were in accordance with the ethical standards of this institute.
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Melicherčík, P., Kotaška, K., Jahoda, D. et al. Antimicrobial peptide in polymethylmethacrylate bone cement as a prophylaxis of infectious complications in orthopedics–an experiment in a murine model. Folia Microbiol 67, 785–791 (2022). https://doi.org/10.1007/s12223-022-00979-0
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DOI: https://doi.org/10.1007/s12223-022-00979-0