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Anhydrobiosis in yeast: role of cortical endoplasmic reticulum protein Ist2 in Saccharomyces cerevisiae cells during dehydration and subsequent rehydration

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Abstract

Two Saccharomyces cerevisiae strains, BY4741 and BY4741-derived strain lacking the IST2 gene (ist2Δ), were used to characterise the possible role of cortical endoplasmic reticulum (ER) protein Ist2 upon cell dehydration and subsequent rehydration. For the first time, we show that not only protein components of the plasma membrane (PM), but also at least one ER membrane protein (Ist2) play an important role in the maintenance of the viability of yeast cells during dehydration and subsequent rehydration. The low viability of the mutant strain ist2∆ upon dehydration-rehydration stress was related to the lack of Ist2 protein in the ER. We revealed that the PM of ist2∆ strain is not able to completely restore its molecular organisation during reactivation from the state of anhydrobiosis. As the result, the permeability of the PM remains high regardless of the type of reactivation (rapid or gradual rehydration). We conclude that ER protein Ist2 plays an important role in ensuring the stability of molecular organisation and functionality of the PM during dehydration-rehydration stress. These results indicate an important role of ER-PM interactions during cells transition into the state of anhydrobiosis and the subsequent restoration of their physiological activities.

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Acknowledgements

The work of ED and AR was supported by University of Latvia grant ZD2016/AZ03. The work of KP and HS was supported by a MEYS Grant Inter-COST LTC20006. This work contributes to the COST Action CA18113 EuroMicropH.

Funding

The work of ED and AR was supported by University of Latvia grant ZD2016/AZ03. The work of KP and HS was supported by a MEYS grant Inter-COST LTC20006. This work contributes to the COST Action CA18113 EuroMicropH.

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Authors and Affiliations

  1. Laboratory of Cell Biology, Institute of Microbiology and Biotechnology, University of Latvia, Jelgavas Str., 1-537, Riga, 1004, Latvia

    Edgars Dauss & Alexander Rapoport

  2. Department of Membrane Transport, Institute of Physiology, Czech Academy of Sciences, Prague, Videnska 1083, 142 20, Prague 4, Czech Republic

    Klára Papoušková & Hana Sychrová

Authors
  1. Edgars Dauss
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  2. Klára Papoušková
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  3. Hana Sychrová
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  4. Alexander Rapoport
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by all authors. The manuscript was written by all authors. All authors read and approved the final version of the manuscript.

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Correspondence to Alexander Rapoport.

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Dauss, E., Papoušková, K., Sychrová, H. et al. Anhydrobiosis in yeast: role of cortical endoplasmic reticulum protein Ist2 in Saccharomyces cerevisiae cells during dehydration and subsequent rehydration. Antonie van Leeuwenhoek 114, 1069–1077 (2021). https://doi.org/10.1007/s10482-021-01578-8

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