Skip to main content
SpringerLink
Log in

MALDI Mass Spectrometry Imaging of Lipids on Free-Floating Brain Sections and Immunohistochemically Colocalized Markers of Neurodegeneration

  • Protocol
  • First Online:
Mass Spectrometry Imaging of Small Molecules

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2437))

Abstract

In mass spectrometry imaging (MSI), the essential steps in sample preparation include collection and storage. The most widely used preservation procedure for MSI consists in freezing samples and storing them at temperatures below −80 °C. On the other hand, the most common method for preserving biological samples in clinical practice is their fixation in paraformaldehyde. The storage of free-floating sections is a particular type of the preservation of paraformaldehyde-fixed tissues that is used in immunohistochemistry. This chapter describes the approach of the multimodal imaging of free-floating brain sections using the MSI of lipids and the immunohistochemistry of neurodegeneration markers.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Buchberger AR, DeLaney K, Johnson J, Li L (2018) Mass spectrometry imaging: a review of emerging advancements and future insights. Anal Chem 90(1):240–265

    Article  CAS  Google Scholar 

  2. Goodwin RJA (2012) Sample preparation for mass spectrometry imaging: small mistakes can lead to big consequences. J Proteome 75(16):4893–4911

    Article  CAS  Google Scholar 

  3. Norris JL, Caprioli RM (2013) Analysis of tissue specimens by matrix-assisted laser desorption/ionization imaging mass spectrometry in biological and clinical research. Chem Rev 113(4):2309–2342

    Article  CAS  Google Scholar 

  4. Schwartz SA, Reyzer ML, Caprioli RM (2003) Direct tissue analysis using matrix-assisted laser desorption/ionization mass spectrometry: practical aspects of sample preparation. J Mass Spectrom 38(7):699–708

    Article  CAS  Google Scholar 

  5. Werner M, Chott A, Fabiano A, Battifora H (2000) Effect of formalin tissue fixation and processing on immunohistochemistry. Am J Surg Pathol 24(7):1016–1019

    Article  CAS  Google Scholar 

  6. Pietrowska M, Gawin M, Polańska J, Widłak P (2016) Tissue fixed with formalin and processed without paraffin embedding is suitable for imaging of both peptides and lipids by MALDI-IMS. Proteomics 16(11-12):1670–1677

    Article  CAS  Google Scholar 

  7. Casadonte R, Kriegsmann M, Zweynert F, Friedrich K, Bretton G, Otto M, Deininger S-O, Paape R, Belau E, Suckau D, Aust D, Pilarsky C, Kriegsmann J (2014) Imaging mass spectrometry to discriminate breast from pancreatic cancer metastasis in formalin-fixed paraffin-embedded tissues. Proteomics 14(7-8):956–964

    Article  CAS  Google Scholar 

  8. Groseclose MR, Massion PP, Chaurand P, Caprioli RM (2008) High-throughput proteomic analysis of formalin-fixed paraffin-embedded tissue microarrays using MALDI imaging mass spectrometry. Proteomics 8(18):3715–3724

    Article  CAS  Google Scholar 

  9. Lemaire R, Desmons A, Tabet JC, Day R, Salzet M, Fournier I (2007) Direct analysis and MALDI imaging of formalin-fixed, paraffin-embedded tissue sections. J Proteome Res 6(4):1295–1305

    Article  CAS  Google Scholar 

  10. Carter CL, McLeod CW, Bunch J (2011) Imaging of phospholipids in formalin fixed rat brain sections by matrix assisted laser desorption/ionization mass spectrometry. J Am Soc Mass Spectrom 22(11):1991

    Article  CAS  Google Scholar 

  11. Harris A, Roseborough A, Mor R, Yeung KKC, Whitehead SN (2020) Ganglioside detection from formalin-fixed human brain tissue utilizing MALDI imaging mass spectrometry. J Am Soc Mass Spectrom 31(3):479–487

    Article  CAS  Google Scholar 

  12. Potts EM, Coppotelli G, Ross JM (2020) Histological-based Stainings using free-floating tissue sections. J Vis Exp (162)

    Google Scholar 

  13. Bachman J (2013) Immunohistochemistry on freely floating fixed tissue sections. Methods Enzymol 533:207–215

    Article  CAS  Google Scholar 

  14. Strnad Š, Pražienková V, Holubová M, Sýkora D, Cvačka J, Maletínská L, Železná B, Kuneš J, Vrkoslav V (2020) Mass spectrometry imaging of free-floating brain sections detects pathological lipid distribution in a mouse model of Alzheimer's-like pathology. Analyst 145(13):4595–4605

    Article  CAS  Google Scholar 

  15. Stoeckli M, Staab D, Wetzel M, Brechbuehl M (2014) iMatrixSpray: a free and open source sample preparation device for mass spectrometric imaging. Chimia (Aarau) 68(3):146–149

    Article  CAS  Google Scholar 

  16. Strnad Š, Pražienková V, Sýkora D, Cvačka J, Maletínská L, Popelová A, Vrkoslav V (2019) The use of 1,5-diaminonaphthalene for matrix-assisted laser desorption/ionization mass spectrometry imaging of brain in neurodegenerative disorders. Talanta 201:364–372

    Article  CAS  Google Scholar 

  17. Sládková K, Houška J, Havel J (2009) Laser desorption ionization of red phosphorus clusters and their use for mass calibration in time-of-flight mass spectrometry. Rapid Commun Mass Spectrom 23(19):3114–3118

    Article  Google Scholar 

Download references

Acknowledgments

This work was supported by project No. 20-00546S of the Czech Science Foundation.

Author information

Authors and Affiliations

  1. Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Prague, Czech Republic

    Štěpán Strnad, Veronika Strnadová, Josef Cvačka, Lenka Maletínská & Vladimír Vrkoslav

  2. University of Chemistry and Technology Prague, Prague, Czech Republic

    David Sýkora

Authors
  1. Štěpán Strnad
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Veronika Strnadová
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. David Sýkora
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Josef Cvačka
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Lenka Maletínská
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Vladimír Vrkoslav
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Vladimír Vrkoslav .

Editor information

Editors and Affiliations

  1. Department of Chemistry, Iowa State University, Ames, IA, USA

    Young-Jin Lee

Rights and permissions

Reprints and permissions

Copyright information

© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Strnad, Š., Strnadová, V., Sýkora, D., Cvačka, J., Maletínská, L., Vrkoslav, V. (2022). MALDI Mass Spectrometry Imaging of Lipids on Free-Floating Brain Sections and Immunohistochemically Colocalized Markers of Neurodegeneration. In: Lee, YJ. (eds) Mass Spectrometry Imaging of Small Molecules. Methods in Molecular Biology, vol 2437. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2030-4_16

Download citation

  • DOI: https://doi.org/10.1007/978-1-0716-2030-4_16

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2029-8

  • Online ISBN: 978-1-0716-2030-4

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation