MMSL 2014, 83(2):67-72 | DOI: 10.31482/mmsl.2014.011

THE USE OF IMMUNOENZYMATIC METHOD FOR DETECTION OF ANTIBODIES AGAINST ZOONOTIC DISEASES IN CZECH SOLDIERS RETURNING FROM AFGHANISTANOriginal article

Petra Straková1,2*, Ivo Rudolf1,2, Oto Pavliš3, Zdeněk Hubálek1,2
1 Institute of Vertebrate Biology, v.v.i., Academy of Sciences of the Czech Republic, Brno
2 Department of Experimental Biology, Faculty of Science, Masaryk University, Brno
3 Military Health Institute, Biological Defence Department, 56166 Těchonín

In this work, we focused on detection of IgG antibodies in the blood sera from Czech soldiers returning from Afghanistan against selected zoonotic diseases by commercially available ELISA kits. Samples were tested for the presence of antibodies against Brucella abortus, Coxiella burnetii, Leptospira spp., complex of Rickettsia conorii, Leishmania infantum, hantaviruses and hepatitis E virus. Except for L. infantum (all persons were negative), we found following seroreactivity rate: 10 % in B. abortus, 11 % in C. burnetii, 20 % in Leptospira spp., 10 % in R. conorii and 4 % in both hepatitis E virus and hantaviruses.

Keywords: ELISA method; zoonoses; soldiers; Afghanistan

Received: May 6, 2014; Revised: May 20, 2014; Published: June 2, 2014  Show citation

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Straková, P., Rudolf, I., Pavliš, O., & Hubálek, Z. (2014). THE USE OF IMMUNOENZYMATIC METHOD FOR DETECTION OF ANTIBODIES AGAINST ZOONOTIC DISEASES IN CZECH SOLDIERS RETURNING FROM AFGHANISTAN. MMSL, 83(2), 67-72. doi: 10.31482/mmsl.2014.011
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