Antitumor and antioxidant activities of purple potato ethanolic extract and its interaction with liposomes, albumin and plasmid DNA

0537129 - ÚFCH JH 2022 RIV GB eng J - Článek v odborném periodiku
Strugata, P. - Urbaniak, A. - Kuryś, P. - Vloch, A. - Ugorski, M. - Hof, Martin - Gabrielska, J.
Antitumor and antioxidant activities of purple potato ethanolic extract and its interaction with liposomes, albumin and plasmid DNA.
Food & Function. Roč. 12, č. 3 (2021), s. 1271-1290. ISSN 2042-6496. E-ISSN 2042-650X
Grant CEP: GA ČR(CZ) GX19-26854X
Institucionální podpora: RVO:61388955
Klíčová slova: antitumor * antioxidant activities * DNA
Obor OECD: Physical chemistry
Impakt faktor: 6.317, rok: 2021
Způsob publikování: Omezený přístup

The aim of study was to broadly determine the biological activities of purple potato ethanolic extract of the Blue Congo variety (BCE). The antioxidative activity of BCE was determined in relation to liposome membranes, and peroxidation was induced by UVB and AAPH. To clarify the antioxidant activity of BCE, we investigated its interactions with hydrophilic and hydrophobic regions of a membrane using fluorimetric and FTIR methods. Next, we investigated the cytotoxicity and pro-apoptotic activities of BCE on two human colon cancer cell lines (HT-29 and Caco-2) and on normal cells (IPEC-J2 ). In addition, the ability to inhibit enzymes which are involved in pro-inflammatory reactions was examined. Furthermore, BCE interactions with serum albumin and plasmid DNA were investigated using steady state fluorescence spectroscopy and single molecule fluorescence technique (TCSPC-FCS). We proved that BCE effectively protects lipid membranes against the process of peroxidation and successfully inhibits the cyclooxygenase and lipoxygenase enzymes. What is more, it interacts with the hydrophilic and hydrophobic parts of lipid membranes as well as with albumin and plasmid DNA. It occurred, that BCE is more cytotoxic against colon cancer cell lines than normal IPEC-J2 cells, it also induces apoptosis in cancer cell lines, but does not induce cell death on normal cells.
Trvalý link: http://hdl.handle.net/11104/0314875