biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 66:39-45, 2022 | DOI: 10.32615/bp.2022.003

The Czech Plant Nucleus Workshop 2021

A. PECINKA1, *, P. PROCHÁZKOVÁ SCHRUMPFOVÁ2, 3, L. FISCHER4, E. DVOŘÁK TOMAŠTÍKOVÁ1, I. MOZGOVÁ5, 6
1 Institute of Experimental Botany of the Czech Academy of Sciences, Centre of the Region Haná for Biotechnological and Agricultural Research, CZ-77900 Olomouc, Czech Republic
2 Laboratory of Functional Genomics and Proteomics, National Centre for Biomolecular Research, Faculty of Science, Masaryk University, CZ-61137, Brno, Czech Republic
3 Mendel Centre for Plant Genomics and Proteomics, Central European Institute of Technology, Masaryk University, CZ-62500, Brno, Czech Republic
4 Department of Experimental Plant Biology, Charles University, Faculty of Science, CZ-12844, Prague, Czech Republic
5 Biology Centre, Czech Acad Sci, Institute of Plant Molecular Biology, CZ-37005, České Budějovice, Czech Republic
6 University of South Bohemia, Faculty of Science, CZ-37005 České Budějovice, Czech Republic

The Czech Plant Nucleus Workshop 2021 (CPNW2021) took place during mid-September 2021 in Olomouc, Czech Republic. About 80 researchers and students working in the field of plant nuclear and chromosome biology in the Czech Republic gathered together to present and discuss their current research. The meeting revealed many plant models that are used to study plant genomes and their organization, and also a great diversity of topics including epigenetic regulation of gene expression, genome stability, telomere biology, or sex chromosomes. CPNW2021 provided a broad platform for establishing new research contacts and collaborations. Here, we summarize the main research directions and findings presented at the CPNW2021 meeting.

Keywords: chromatin, chromosome, DNA damage repair, DNA methylation, environmental responses, nucleus, sex- and B-chromosomes.

Received: December 14, 2021; Revised: December 14, 2021; Accepted: February 8, 2022; Published online: February 25, 2022  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
PECINKA, A., PROCHÁZKOVÁ SCHRUMPFOVÁ, P., FISCHER, L., DVOŘÁK TOMAŠTÍKOVÁ, E., & MOZGOVÁ, I. (2022). The Czech Plant Nucleus Workshop 2021. Biologia plantarum, 66, Article 39-45. https://doi.org/10.32615/bp.2022.003
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Adamus, M., Lelkes, E., Potesil, D., Ganji, S.R., Kolesar, P., Zabrady, K., Zdrahal, Z., Palecek, J.J.: Molecular insights into the architecture of the human SMC5/6 complex. - J. mol. Biol. 432: 3820-3837, 2020. Go to original source...
    2. Ariel, F., Jegu, T., Latrasse, D., Romero-Barrios, N., Christ, A., Benhamed, M., Crespi, M.: Noncoding transcription by alternative RNA polymerases dynamically regulates an auxin-driven chromatin loop. - Mol. Cells 55: 383-396, 2014. Go to original source...
    3. Bieluszewski, T., Xiao, J., Yang, Y., Wagner, D.: PRC2 activity, recruitment, and silencing: a comparative perspective. - Trends Plant Sci. 26: 1186-1198, 2021. Go to original source...
    4. Blavet, N., Yang, H., Su, H.D., Solansky, P., Douglas, R.N., Karafiatova, M., Simkova, L., Zhang, J., Liu, Y., Hou, J., Shi, X.W., Chen, C., El-Walid, M., McCaw, M.E., Albert, P.S., Gao, Z., Zhao, C.Z., Ben-Zvi, G., Glick, L., Kol, G., Shi, J.H., Vrana, J., Simkova, H., Lamb, J.C., Newton, K., Dawe, R.K., Dolezel, J., Ji, T.M., Baruch, K., Cheng, J.L., Han, F.P., Birchler, J.A., Bartos, J.: Sequence of the supernumerary B chromosome of maize provides insight into its drive mechanism and evolution. - Proc. nat. Acad. Sci. USA 118: e2104254118, 2021. Go to original source...
    5. Čermák, V., Tyč, D., Přibylová, A., Fischer, L.: Unexpected variations in posttranscriptional gene silencing induced by differentially produced dsRNAs in tobacco cells. - Biochim. biophys. Acta - Gene Regul. Mech. 1863: 194647, 2020. Go to original source...
    6. Díaz, M., Pecinka, A.: Scaffolding for repair: understanding molecular functions of the SMC5/6 complex. - Genes (Basel) 9: 36, 2018. Go to original source...
    7. Dokládal, L., Benková, E., Honys, D., Dupľáková, N., Lee, L.-Y., Gelvin, S.B., Sýkorová, E.: An armadillo-domain protein participates in a telomerase interaction network. - Plant mol. Biol. 97: 407-420, 2018. Go to original source...
    8. Fajkus, P., Peška, V., Závodník, M., Fojtová, M., Fulnečková, J., Dobias, Š., Kilar, A., Dvořáčková, M., Zachová, D., Nečasová, I., Sims, J., Sýkorová, E., Fajkus, J.: Telomerase RNAs in land plants. - Nucl. Acids Res. 47: 9842-9856, 2019. Go to original source...
    9. Fajkus, P., Kilar, A., Nelson, A.D.L., Holá, M., Peška, V., Goffová, I., Fojtová, M., Zachová, D., Fulnečková, J., Fajkus, J.: Evolution of plant telomerase RNAs: farther to the past, deeper to the roots. - Nucl. Acids Res. 49: 7680-7694, 2021. Go to original source...
    10. Franek, M., Kilar, A., Fojtík, P., Olšinová, M., Benda, A., Rotrekl, V., Dvořáčková, M., Fajkus, J.: Super-resolution microscopy of chromatin fibers and quantitative DNA methylation analysis of DNA fiber preparations. - J. Cell Sci. 134: jcs258374, 2021. Go to original source...
    11. Fransz, P., De Jong, J.H., Lysak, M., Castiglione, M.R., Schubert, I.: Interphase chromosomes in Arabidopsis are organized as well defined chromocenters from which euchromatin loops emanate. - Proc. nat. Acad. Sci. USA 99: 14584-14589, 2002. Go to original source...
    12. Glombik, M., Bačovský, V., Hobza, R., Kopecký, D.: Competition of parental genomes in plant hybrids. - Front. Plant Sci. 11: 200, 2020. Go to original source...
    13. Glombik, M., Copetti, D., Bartos, J., Stoces, S., Zwierzykowski, Z., Ruttink, T., Wendel, J.F., Duchoslav, M., Dolezel, J., Studer, B., Kopecky, D.: Reciprocal allopolyploid grasses (Festuca × Lolium) display stable patterns of genome dominance. - Plant J. 107: 1166-1182, 2021. Go to original source...
    14. Hobza, R., Hudzieczek, V., Kubat, Z., Cegan, R., Vyskot, B., Kejnovsky, E., Janousek, B.: Sex and the flower - developmental aspects of sex chromosome evolution. - Ann. Bot. 122: 1085-1101, 2018. Go to original source...
    15. Holá, M., Vágnerová, R., Angelis, K.J.:. Kleisin NSE4 of the SMC5/6 complex is necessary for DNA double strand break repair, but not for recovery from DNA damage in physcomitrella (Physcomitrium patens). - Plant mol. Biol. 107: 355-364, 2021. Go to original source...
    16. Jesionek, W., Bodláková, M., Kubát, Z., Čegan, R., Vyskot, B., Vrána, J., Šafář, J., Puterova, J., Hobza, R.: Fundamentally different repetitive element composition of sex chromosomes in Rumex acetosa. - Ann. Bot. 127: 33-47, 2021. Go to original source...
    17. Karafiátová, M., Bednářová, M., Said, M., Čížková, J., Holušová, K., Blavet, N., Bartoš, J.: The B chromosome of Sorghum purpureosericeum reveals the first pieces of its sequence. - J. exp. Bot. 72: 1606-1616, 2021. Go to original source...
    18. Kim, J., Bordiya, Y., Kathare, P.K., Zhao, B., Zong, W., Huq, E., Sung, S.: Phytochrome B triggers light-dependent chromatin remodelling through the PRC2-associated PHD finger protein VIL1. - Nat. Plants 7: 1213-1219, 2021. Go to original source...
    19. Kolářová, K., Nešpor Dadejová, M., Loja, T., Lochmanová, G., Sýkorová, E., Dvořáčková, M.: Disruption of NAP1 genes in Arabidopsis thaliana suppresses the fas1 mutant phenotype, enhances genome stability and changes chromatin compaction. - Plant J. 106: 56-73, 2021. Go to original source...
    20. Kovacik, M., Nowicka, A., Pecinka, A.: Isolation of high purity tissues from developing barley seeds. - J. visualized Exp. 164: e61681, 2020. Go to original source...
    21. Kubalová, I., Câmara, A.S., Cápal, P., Beseda, T., Rouillard, J., Krause, G.M., Toegelová, H., Himmelbach, A., Stein, N., Houben, A., Doležel, J., Mascher, M., Šimková, H., Schubert, V.: Helical metaphase chromatid coiling is conserved. - bioRxiv: 2021.09.16.460607, 2021a.
    22. Kubalová, I., Němečková, A., Weisshart, K., Hřibová, E., Schubert, V.: Comparing super-resolution microscopy techniques to analyze chromosomes. - Int. J. mol. Sci. 22: 1903, 2021b. Go to original source...
    23. Majerová, E., Mandáková, T., Vu, G.T.H., Fajkus, J., Lysak, M.A., Fojtová, M.: Chromatin features of plant telomeric sequences at terminal vs. internal positions. - Front. Plant Sci. 5: 1-10, 2014. Go to original source...
    24. Němečková, A., Koláčková, V., Vrána, J., Doležel, J., Hřibová, E.: DNA replication and chromosome positioning throughout the interphase in three-dimensional space of plant nuclei. - J. exp. Bot. 71: 6262-6272, 2020. Go to original source...
    25. Novák, P., Guignard, M.S., Neumann, P., Kelly, L.J., Mlinarec, J., Koblížková, A., Dodsworth, S., Kovařík, A., Pellicer, J., Wang, W., Macas, J., Leitch, I.J., Leitch, A.R.: Repeat-sequence turnover shifts fundamentally in species with large genomes. - Nat. Plants 6: 1325-1329, 2020. Go to original source...
    26. Nowicka, A., Kovacik, M., Tokarz, B., Vrána, J., Zhang, Y., Weigt, D., Doležel, J., Pecinka, A.: Dynamics of endoreduplication in developing barley seeds. - J. exp. Bot. 72: 268-282, 2021. Go to original source...
    27. Perničková, K., Koláčková, V., Lukaszewski, A.J., Fan, Ch., Vrána, J., Duchoslav, M., Jenkins, G., Phillips, D., Šamajová, O., Sedlářová, M., Šamaj, J., Doležel, J., Kopecký, D.: Instability of alien chromosome introgressions in wheat associated with improper positioning in the nucleus. - Int. J. mol. Sci. 20: 1448, 2019. Go to original source...
    28. Peska, V., Garcia, S.: Origin, diversity, and evolution of telomere sequences in plants. - Front. Plant Sci. 11: 117, 2020. Go to original source...
    29. Peska, V., Prochazkova Schrumpfova, P., Fajkus, J.:Using the Telobox to search for plant telomere binding proteins. - Curr. Protein Pept. Sci. 12: 75-83, 2011. Go to original source...
    30. Přibylová, A., Čermák, V., Tyč, D., Fischer, L.: Detailed insight into the dynamics of the initial phases of de novo RNA-directed DNA methylation in plant cells. - Epigenetics Chromatin 12: 54, 2019. Go to original source...
    31. Procházková, K., Finke, A., Dvořák Tomaštíková, E., Filo, J., Bente, H., Dvořák, P., Ovečka, M., Šamaj, J., Pecinka, A.:. Zebularine induces enzymatic DNA-protein crosslinks in 45S rDNA heterochromatin of Arabidopsis nuclei. - Nucl. Acids Res. 50: 244-258, 2022. Go to original source...
    32. Procházková Schrumpfová, P., Fojtová, M., Fajkus, J.: Telomeres in plants and humans: not so different, not so similar. - Cells 8: 58, 2019. Go to original source...
    33. Rabl, C.: Über Zelltheilung. - Morphologisches Jahrbuch 10: 214-330, 1885.
    34. Richards, E.J., Ausubel, F.M.: Isolation of a higher eukaryotic telomere from Arabidopsis thaliana. - Cell 53: 127-136, 1988. Go to original source...
    35. Rodríguez Lorenzo, J.L., Hubinský, M., Vyskot, B., Hobza, R.: Histone post-translational modifications in Silene latifolia X and Y chromosomes suggest a mammal-like dosage compensation system. - Plant Sci. 299: 110528, 2020. Go to original source...
    36. Schrumpfová, P.P., Fajkus, J.: Composition and function of Telomerase-A polymerase associated with the origin of Eukaryotes. - Biomolecules 10: 1425, 2020. Go to original source...
    37. Schrumpfová, P.P., Vychodilová, I., Dvořáčková, M., Majerská, J., Dokládal, L., Schořová, S., Fajkus, J.: Telomere repeat binding proteins are functional components of Arabidopsis telomeres and interact with telomerase. - Plant J. 77: 770-781, 2014. Go to original source...
    38. Shan, W., Kubová, M., Mandáková, T., Lysak, M.A.: Nuclear organization in crucifer genomes: nucleolus-associated telomere clustering is not a universal interphase configuration in Brassicaceae. - Plant J. 108: 528-540, 2021. Go to original source...
    39. Sharaf, A., Vijayanathan, M., Oborník, M., Mozgová, I.: Phylogenetic profiling suggests early origin of the core subunits of Polycomb Repressive Complex 2 (PRC2). - bioRxiv: 2021.07.16.452543, 2021. Go to original source...
    40. Shen, Q., Lin, Y., Li, Y., Wang, G.: Dynamics of H3K27me3 modification on plant adaptation to environmental cues. - Plants (Basel) 10: 1165, 2021. Go to original source...
    41. Stingele, J., Jentsch, S.: DNA-protein crosslink repair. - Nat. Rev. mol. cell. Biol. 16: 455-460, 2015. Go to original source...
    42. Strejčková, B., Čegan, R., Pecinka, A., Milec, Z., Šafář, J.: Identification of polycomb repressive complex 1 and 2 core components in hexaploid bread wheat. - BMC Plant Biol. 20: 175, 2020. Go to original source...
    43. Strejčková, B., Milec, Z., Holušová, K., Cápal, P., Vojtková, T., Čegan, R., Šafář, J.: In-depth sequence analysis of bread wheat VRN1 genes. - Int. J. mol. Sci. 22: 12284, 2021. Go to original source...
    44. Uchida, W., Matsunaga, S., Sugiyama, R., Kawano, S.: Interstitial telomere-like repeats in the Arabidopsis thaliana genome. - Genes Genet. Syst. 77: 63-67, 2002. Go to original source...
    45. Wiese, A.J., Steinbachová, L., Timofejeva, L., Čermák, V., Klodová, B., Ganji, R.S., Limones-Mendez, M., Bokvaj, P., Hafidh, S., Potěšil, D., Honys, D.: Arabidopsis bZIP18 and bZIP52 accumulate in nuclei following heat stress where they regulate the expression of a similar set of genes. - Int. J. mol. Sci. 22: 530, 2021. Go to original source...
    46. Xu, S., Chong, K.: Remembering winter through vernalisation. - Nat. Plants 4: 997-1009, 2018. Go to original source...
    47. Yang, F., Fernández-Jiménez, N., Tučková, M., Vrána, J., Cápal, P., Díaz, M., Pradillo, M., Pecinka, A.: Defects in meiotic chromosome segregation lead to unreduced male gametes in Arabidopsis SMC5/6 complex mutants. - Plant Cell 33: 3104-3119 2021. Go to original source...
    48. Yokoyama, R., Hirakawa, T., Hayashi, S., Sakamoto, T., Matsunaga, S.: Dynamics of plant DNA replication based on PCNA visualization. - Nat. Publ. 6: 29657, 2016. Go to original source...
    49. Zhang, H., Lang, Z., Zhu, J.-K.: Dynamics and function of DNA methylation in plants. - Nat. Rev. Mol. Cell Biol. 19: 489-506, 2018. Go to original source...

    Return to the content