Functionalized hydrogel microparticles prepared by microfluidics and their interaction with tumour marker carbonic anhydrase IX

A. Pittermannová; Z. Ruberová; D. Lizoňová; A. Hubatová-Vacková; O. Kašpar; A. Zadražil; V. Král; M. Pechar; R. Pola; J. Bibette; N. Bremond; F. Štěpánek; V. Tokárová

doi:10.1039/D0SM01018A

Functionalized hydrogel microparticles prepared by microfluidics and their interaction with tumour marker carbonic anhydrase IX†

A. Pittermannová,^ab Z. Ruberová,^a D. Lizoňová,^a A. Hubatová-Vacková,^a O. Kašpar,

^a A. Zadražil,^a V. Král,^c M. Pechar,

^d R. Pola,^d J. Bibette,^b N. Bremond,

^b F. Štěpánek^a and V. Tokárová

*^a

Author affiliations

* Corresponding authors

^a Department of Chemical Engineering, University of Chemistry and Technology, Technická 3, 166 28 Prague 6, Czech Republic
E-mail: viola.tokarova@vscht.cz

^b Laboratory Colloids and Divided Matter – Chemistry, Biology and Innovation (CBI) UMR8231, ESPCI Paris, CNRS, PSL Research University, 10 rue Vauquelin, Paris, France

^c Laboratory of Structural Biology, Institute of Molecular Genetics of the Czech Academy of Sciences, Vídeňská 1083, 142 20 Prague 4, Czech Republic

^d Laboratory of Biomedical Polymers, Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského Nám. 2, 162 06 Prague 6, Czech Republic

Abstract

Microfluidics allows precise control of the synthesis of microparticles for specific applications, where size and morphology play an important role. In this work, we have introduced microfluidic chip design with dedicated extraction and gelation sections allowing to prepare hydrogel particles in the size range of a red blood cell. The influence of the extractive channel size, alginate concentration and type of storage media on the final size of the prepared alginate microparticles has been discussed. The second part of the work is dedicated to the surface modification of prepared particles using chitosan, pHPMA and the monoclonal antibody molecule, IgG M75. The specific interaction of the antibody molecule with an antigen domain of carbonic anhydrase IX, the transmembrane tumour protein associated with several types of cancer, is demonstrated by fluorescence imaging and compared to an isotypic antibody molecule.

Soft Matter

Functionalized hydrogel microparticles prepared by microfluidics and their interaction with tumour marker carbonic anhydrase IX†

Abstract

Supplementary files

Article information

Download Citation

Permissions

Functionalized hydrogel microparticles prepared by microfluidics and their interaction with tumour marker carbonic anhydrase IX

Social activity

Search articles by author

Spotlight

Advertisements