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Protein profiling of SH-SY5Y neuroblastoma cells: The effect of rhein

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Abstract

4,5-Dihydroxyanthraquinone-2-carboxylic acid (Rhein) has been shown to have various physiological and pharmacological properties including anticancer activity and modulatory effects on bioenergetics. In this study, we explored the impact of rhein on protein profiling of undifferentiated (UC) and differentiated (DC) SH-SY5Y cells. Besides that, the cellular morphology and expression of differentiation markers were investigated to determine the effect of rhein on retinoic acid-induced neuronal cell differentiation. Using two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry we evaluated the changes in the proteome of both UC and DC SH-SY5Y cells after 24 h treatment with rhein. Validation of selected differentially expressed proteins and the assessment of neuronal differentiation markers were performed by western blotting. Proteomic analysis revealed significant changes in the abundance of 15 proteins linked to specific cellular processes such as cytoskeleton structure and regulation, mitochondrial function, energy metabolism, protein synthesis and neuronal plasticity. We also observed that the addition of rhein to the cultured cells during differentiation resulted in a significantly reduced neurite outgrowth and decreased expression of neuronal markers. These results indicate that rhein may strongly interfere with the differentiation process of SH-SY5Y neuroblastoma cells and is capable of inducing marked proteomic changes in these cells.

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Abbreviations

GAP43:

growth-associated protein 43

MAP2:

microtubule-associated protein 2

NSE:

neuron-specific enolase

PCNA:

proliferating cell nuclear antigen

PRX:

peroxiredoxin

RA:

retinoic acid

SNP:

synaptophysin

TH:

tyrosine hydroxylase

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Acknowledgements

This study was supported by the Czech Science Foundation (16-21228Y), by the Charles University Grant Agency (898616), by the project no. LQ1605 (MEYS CR, NPU II) and institutional project SVV-260434/2018. PT was a recipient of Primus/SCI/33 grant from Charles University. We appreciate the professional assistance of Karel Harant and Pavel Talacko (Proteomics Core Facility of BIOCEV, Faculty of Science, Charles University) with mass spectrometric analysis.

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Authors and Affiliations

  1. Department of Physiology, Faculty of Science, Charles University, Prague, Czech Republic

    Zuzana Cockova, Hana Ujcikova, Petr Telensky & Jiri Novotny

  2. Department of Biomathematics, Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic

    Hana Ujcikova

  3. International Clinical Research Center, St. Anne’s University Hospital Brno, Brno, Czech Republic

    Petr Telensky

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  1. Zuzana Cockova
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  2. Hana Ujcikova
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Cockova, Z., Ujcikova, H., Telensky, P. et al. Protein profiling of SH-SY5Y neuroblastoma cells: The effect of rhein. J Biosci 44, 88 (2019). https://doi.org/10.1007/s12038-019-9908-0

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