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Fabrication of a glycerol-citrate polymer coated tricalcium phosphate bone cements: Structural investigation and material properties

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Abstract

Calcium phosphate cements (CPCs) belong to one of the most prominent biomaterials used for filling and regeneration of hard tissues, however poor mechanical and biological properties limit their widespread use in some clinical applications. To solve these problems, a biodegradable glycerol-citrate (G-CA) polyester was synthesized and coated on tricalcium phosphate cement (TCP) powders in amounts up to 10 wt% of the G-CA content using a simple solution infiltration technique in ethanol solution. Chemical and structural analysis of the G-CA elastomer and TCP/G-CA composites were thoroughly analyzed with chromatographic techniques, solid-state nuclear magnetic resonance (ssNMR), differential scanning calorimetry and thermogravimetry (DSC/TG), X-ray diffraction (XRD), Fourier-Transform Infrared spectroscopy (FTIR) and field emission scanning electron microscopy (SEM). The results demonstrated that the incorporation of 2.5 and 5 wt% of G-CA into TCP cement led to a significant increase of mechanical strength of the cements due to the formation of thin and homogeneous elastomer coating on cement particles, reinforcing the microstructure through hydrogen bonds between residual COOH groups of polymer and surface phosphate groups of the cement matrix. Additional in vitro testing of extracts cytotoxicity revealed a high proliferation of osteoblasts in all composites, demonstrating a promising application potential in regenerative medicine.

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Acknowledgements

This work was supported by the Slovak Grant Agency of the Ministry of Education of the Slovak Republic and the Slovak Academy of Sciences, Project No. 2/0034/21 and the Slovak Research and Development Agency under the contract No. APVV-17-0110.

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Authors and Affiliations

  1. Institute of Materials Research of SAS, Watsonova 47, 04001, Kosice, Slovak Republic

    T. Sopcak, L. Medvecky, M. Giretova, R. Stulajterova, F. Kromka & M. Faberova

  2. Institute of Macromolecular Chemistry of the Czech Academy of Sciences, Heyrovskeho nam. 2, 162 06, Prague 6, Czech Republic

    J. Brus & M. Urbanova

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  1. T. Sopcak
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Correspondence to T. Sopcak.

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10965_2021_2596_MOESM1_ESM.jpg

Supplementary file1 Fig.s1 (Supplementary material) GPC chromatogram of the synthesized G_CA elastomer with the relevant calibration points (JPG 390 KB)

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Sopcak, T., Medvecky, L., Giretova, M. et al. Fabrication of a glycerol-citrate polymer coated tricalcium phosphate bone cements: Structural investigation and material properties. J Polym Res 28, 231 (2021). https://doi.org/10.1007/s10965-021-02596-w

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