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Degradation of phospholipids under different types of irradiation and varying oxygen saturation

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Abstract

The effects of different types of radiation on the formation of peroxide forms of 2-dioleoyl-sn-glycero-3-phosphocholine were studied under various conditions. For the irradiation, an aqueous solution of small unilamellar vesicles was prepared. Variations in parameters such as the dose rate and molecular oxygen saturation levels were evaluated. Our study suggests that the mechanism of the peroxides formation process remains unchanged under irradiation by accelerated electrons, gamma and accelerated protons. The values of radiation chemical yields of the peroxidic form depend on the type of radiation, dose rate, and the saturation of molecular oxygen. The level of oxygen saturation strongly affects the values of radiation chemical yields as well, as the dissolved oxygen is an important agent participating in peroxidation and it is a source of free radicals during the radiolysis. The values of radiation chemical yields strongly suggest that the mechanism of radiation-induced peroxidation of phosphatidylcholines does not proceed via chain reaction.

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Acknowledgements

The authors would like to acknowledge the support by the Czech Science Foundation (Grant No. 13-28721S). The irradiation took place at the Center of Accelerators and Nuclear Analytical Methods (CANAM, project No. LM2011019) supported by the MEYS CR. M.D. acknowledges financial support provided by the Czech Science foundation (Grant No. P108/12/G108).

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Authors and Affiliations

  1. Institute of Physics of the CAS, Na Slovance 1999/2, 182 21, Prague, Czech Republic

    Luděk Vyšín & Libor Juha

  2. FNSPE, Czech Technical University in Prague, Břehová 7, 115 19, Prague, Czech Republic

    Luděk Vyšín, Kateřina Tomanová, Tereza Pavelková, Viliam Múčka & Václav Čuba

  3. Nuclear Physics Institute of the CAS, Husinec-Řež 130, 250 68, Řež, Czech Republic

    Richard Wagner & Marie Davídková

  4. Institute of Plasma Physics of the CAS, Za Slovankou 3, 182 00, Prague, Czech Republic

    Libor Juha

Authors
  1. Luděk Vyšín
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  2. Kateřina Tomanová
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  3. Tereza Pavelková
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  4. Richard Wagner
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  5. Marie Davídková
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  6. Viliam Múčka
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  7. Václav Čuba
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  8. Libor Juha
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Correspondence to Luděk Vyšín.

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Funding

This study was funded by the Czech Science Foundation (Grant No. GA 13-28721S and P108/12/G108), Ministry of Education Youth and Sport, CR (project n. LM 2011019).

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Vyšín, L., Tomanová, K., Pavelková, T. et al. Degradation of phospholipids under different types of irradiation and varying oxygen saturation. Radiat Environ Biophys 56, 241–247 (2017). https://doi.org/10.1007/s00411-017-0693-6

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  • DOI: https://doi.org/10.1007/s00411-017-0693-6

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