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Peptidomics and Capillary Electrophoresis

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Separation Techniques Applied to Omics Sciences

Part of the book series: Advances in Experimental Medicine and Biology ((PMISB,volume 1336))

Abstract

Peptides play a crucial role in many vitally important functions of living organisms. The goal of peptidomics is the identification of the “peptidome,” the whole peptide content of a cell, organ, tissue, body fluid, or organism. In peptidomic or proteomic studies, capillary electrophoresis (CE) is an alternative technique for liquid chromatography. It is a highly efficient and fast separation method requiring extremely low amounts of sample. In peptidomic approaches, CE is commonly combined with mass spectrometric (MS) detection. Most often, CE is coupled with electrospray ionization MS and less frequently with matrix-assisted laser desorption/ionization MS. CE-MS has been employed in numerous studies dealing with determination of peptide biomarkers in different body fluids for various diseases, or in food peptidomic research for the analysis and identification of peptides with special biological activities. In addition to the above topics, sample preparation techniques commonly applied in peptidomics before CE separation and possibilities for peptide identification and quantification by CE-MS or CE-MS/MS methods are discussed in this chapter.

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Abbreviations

AD:

Alzheimer disease

BGE:

background electrolyte

BLAST:

basic local alignment search tool

BPE:

base peak electropherogram

CE:

capillary electrophoresis

CID:

collision-induced dissociation

CKD:

chronic kidney disease

CSF:

cerebrospinal fluid

ECD:

electron capture dissociation

EIE:

extracted ion electropherogram

EOF:

electroosmotic flow

ESI:

electrospray ionization

ETD:

electron transfer dissociation

FDR:

false discovery rate

FT-ICR:

Fourier-transform ion cyclotron resonance

HCD:

high-energy collisional dissociation

IEF:

isoelectric focusing

ITP:

isotachophoresis

IT:

ion trap

LC:

liquid chromatography

MALDI:

matrix-assisted laser desorption ionization

MRM:

multiple reaction monitoring

MS:

mass spectrometry

MS/MS:

tandem mass spectrometry

PTM:

posttranslational modification

RP:

reversed-phase

SDS:

sodium dodecyl sulfate

SPE:

solid-phase extraction

SPME:

solid-phase microextraction

SRM:

selected reaction monitoring

TOF:

time of flight

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Acknowledgments

The work was supported by the Czech Science Foundation, grant no. 20-03899S, and by the Czech Academy of Sciences, research project RVO 61388963. The authors thank Dr. Petra Sázelová for her help in preparation of this manuscript.

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The authors declare no conflict of interest.

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  1. Institute of Organic Chemistry and Biochemistry, The Czech Academy of Sciences, Prague, Czechia

    Sille Štěpánová & Václav Kašička

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  1. Analytical Chemistry Department, University of Campinas, Institute of Chemistry, Campinas, São Paulo, Brazil

    Ana Valéria Colnaghi Simionato

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Štěpánová, S., Kašička, V. (2021). Peptidomics and Capillary Electrophoresis. In: Colnaghi Simionato, A.V. (eds) Separation Techniques Applied to Omics Sciences. Advances in Experimental Medicine and Biology(), vol 1336. Springer, Cham. https://doi.org/10.1007/978-3-030-77252-9_5

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