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Mechanistic insights into the Orai channel by molecular dynamics simulations
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SYSNO ASEP 0519579 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Mechanistic insights into the Orai channel by molecular dynamics simulations Author(s) Bonhenry, Daniel (MBU-M) ORCID
Schober, R. (AT)
Schmidt, T. (AT)
Waldherr, L. (AT)
Ettrich, Rüdiger (MBU-M)
Schindel, R. (AT)Source Title Seminars in Cell & Developmental Biology. - : Elsevier - ISSN 1084-9521
Roč. 94, October 2019 SI (2019), s. 50-58Number of pages 9 s. Language eng - English Country GB - United Kingdom Keywords Molecular dynamics ; MD-simulations ; Orai Subject RIV BO - Biophysics OECD category Biophysics R&D Projects GA13-21053S GA ČR - Czech Science Foundation (CSF) LM2015055 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2015055 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LTC17069 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Research Infrastructure C4SYS - 90055 - Mikrobiologický ústav AV ČR, v. v. i. Method of publishing Limited access Institutional support MBU-M - RVO:61388971 UT WOS 000485875300007 EID SCOPUS 85059862388 DOI 10.1016/j.semcdb.2019.01.002 Annotation Highly Ca2+ selective channels trigger a large variety of cellular signaling processes in both excitable and non-excitable cells. Among these channels, the Orai channel is unique in its activation mechanism and its structure. It mediates Ca2+ influx into the cytosol with an extremely small unitary conductance over longer time-scales, ranging from minutes up to several hours. Its activation is regulated by the Ca2+ content of the endoplasmic reticulum (ER). Depletion of luminal [Ca2+](ER) is sensed by the STIM1 single transmembrane protein that directly binds and gates the Orail channel. Orai mediated Ca2+ influx increases cytosolic Ca2+ from 100 nM up to low micromolar range close to the pore and thereby forms Ca2+ microdomains. Hence, these features of the Orai channel can trigger long-term signaling processes without affecting the overall Ca2+ content of a single living cell. Here we focus on the architecture and dynamic conformational changes within the Orai channel. This review summarizes current achievements of molecular dynamics simulations in combination with live cell recordings to address gating and permeation of the Orai channel with molecular precision. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2020 Electronic address https://www.sciencedirect.com/science/article/pii/S1084952118300375?via%3Dihub
Number of the records: 1