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Biocontrol of Soft Rot: Confocal Microscopy Highlights Virulent Pectobacterial Communication and Its Jamming by Rhodococcal Quorum-Quenching
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SYSNO ASEP 0507905 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Biocontrol of Soft Rot: Confocal Microscopy Highlights Virulent Pectobacterial Communication and Its Jamming by Rhodococcal Quorum-Quenching Author(s) Chane, A. (FR)
Barbey, C. (FR)
Robert, M. (FR)
Merieau, A. (FR)
Konto-Ghiorghi, Y. (FR)
Beury-Cirou, A. (FR)
Feuilloley, M. (FR)
Pátek, Miroslav (MBU-M) RID, ORCID
Gobert, V. (FR)
Latour, X. (FR)Source Title Molecular Plant-Microbe Interactions. - : American Phytopathological Society - ISSN 0894-0282
Roč. 32, č. 7 (2019), s. 802-812Number of pages 11 s. Language eng - English Country US - United States Keywords green fluorescent protein ; lactone-mediated communication ; n-acylhomoserine lactones Subject RIV EE - Microbiology, Virology OECD category Microbiology Method of publishing Limited access Institutional support MBU-M - RVO:61388971 UT WOS 000473639600003 EID SCOPUS 85066430113 DOI 10.1094/MPMI-11-18-0314-R Annotation Confocal laser-scanning microscopy was chosen to observe the colonization and damage caused by the soft rot Pectobacterium atrosepticum and the protection mediated by the biocontrol agent Rhodococcus erythropolis. We developed dual-color reporter strains suited for monitoring quorum-sensing and quorum-quenching activities leading to maceration or biocontrol, respectively. A constitutively expressed cyan or red fluorescent protein served as a cell tag for plant colonization, while an inducible expression reporter system based on the green fluorescent protein gene enabled the simultaneous recording of signaling molecule production, detection, or degradation. The dual-colored pathogen and biocontrol strains were used to coinoculate potato tubers. At cellular quorum, images revealed a strong pectobacterial quorum-sensing activity, especially at the plant cell walls, as well as a concomitant rhodococcal quorum-quenching response, at both the single-cell and microcolony levels. The generated biosensors appear to be promising and complementary tools useful for molecular and cellular studies of bacterial communication and interference. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2020 Electronic address https://apsjournals.apsnet.org/doi/10.1094/MPMI-11-18-0314-R
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