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Retinoic acid improves maturation rate and upregulates the expression of antioxidant-related genes in in vitro matured buffalo (Bubalus bubalis) oocytes
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SYSNO ASEP 0506864 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Retinoic acid improves maturation rate and upregulates the expression of antioxidant-related genes in in vitro matured buffalo (Bubalus bubalis) oocytes Author(s) Gad, Ahmed (UZFG-Y) ORCID
Hamed, S. A. (EG)
Khalifa, M. (EG)
Amin, A. (EG)
El-Sayed, A. (EG)
Swiefy, S. A. (EG)
El-Assal, S. (EG)Source Title International journal of veterinary science and medicine. - : Elsevier - ISSN 2314-4599
Roč. 6, č. 2 (2018), s. 279-285Number of pages 7 s. Publication form Print - P Language eng - English Country EC - Ecuador Keywords antioxidant genes ; cleavage rate ; follicular fluid Subject RIV EB - Genetics ; Molecular Biology OECD category Developmental biology Method of publishing Open access Institutional support UZFG-Y - RVO:67985904 UT WOS 000451781200024 DOI 10.1016/j.ijvsm.2018.09.003 Annotation Retinoic acid, vitamin A metabolite, plays a role in oocyte development and maturation in different ways including gene expression alteration and/or prohibiting oxidative stress. The objective of this study was to examine the effect of 9-cis-retinoic acid (9-cisRA) on the quality and maturation rate of buffalo oocytes. Cumulusoocyte complexes (COCs, n = 460) were collected from ovaries of slaughtered buffalos. Varying concentrations of 9-cisRA (0, 5, 50, and 200 nM) were added to the maturation medium, and the following parameters were analyzed: (i) maturation and cleavage rates, (ii) mitochondrial activity and reactive oxygen species (ROS) levels, (iii) expression level of antioxidant-related genes (PRDX1, SOD1, CAT, HOMX1, and GPX4) using RT-qPCR. Maturation rate was significantly improved in 5 nM 9-cisRA oocyte group (95.8%, P < .05) compared to control and other treatment groups (86.7% in control group). The same oocyte group exhibited significantly higher mitochondrial membrane potential activity and lower ROS accumulation level compared to other treatment groups. Antioxidant-related genes were up-regulated in oocytes matured with 5 or 50 nM 9-cisRA compared to control and 200 nM 9-cisRA groups. In contrast, 200 nM of 9-cisRA showed a clear down-regulation for antioxidant-related genes except for PRDX1. In conclusion, supplementation of 9-cisRA with a lower concentration (5 nM) to the buffalo oocytes maturation media promotes maturation rate through a protection mechanism that maintains adequate levels of antioxidant-related transcripts and improves mitochondrial activity. However, 9cisRA has no significant effect on the cleavage rate of the treated oocytes. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2020 Electronic address https://www.tandfonline.com/doi/full/10.1016/j.ijvsm.2018.09.003
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