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The Timing and Characterization of Maternal to Zygote Transition and Mid-Blastula Transition in Sterlet Acipenser ruthenus and A. ruthenus x Acipenser gueldenstaedtii Hybrid
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SYSNO ASEP 0503711 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The Timing and Characterization of Maternal to Zygote Transition and Mid-Blastula Transition in Sterlet Acipenser ruthenus and A. ruthenus x Acipenser gueldenstaedtii Hybrid Author(s) Pocherniaieva, K. (CZ)
Güralp, H. (CZ)
Saito, T. (CZ)
Pšenička, M. (CZ)
Tichopád, T. (CZ)
Janko, Karel (UZFG-Y) RID, ORCID
Kašpar, V. (CZ)Source Title Turkish Journal of Fisheries and Aquatic Sciences. - : Central Fisheries Research Institute - ISSN 1303-2712
Roč. 19, č. 2 (2019), s. 167-174Number of pages 8 s. Publication form Print - P Language eng - English Country TR - Turkey Keywords cell cycle ; DAPI ; synchronization Subject RIV EG - Zoology OECD category Zoology R&D Projects GA17-09807S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support UZFG-Y - RVO:67985904 UT WOS 000577400900009 EID SCOPUS 85053340233 DOI 10.4194/1303-2712-v19_2_09 Annotation Detailed knowledge of early embryo development is a prerequisite for implementation of reproductive biotechnologies. We described the timing of mid-blastula transition in the sturgeon Acipenser ruthenus and hybrid of A. ruthenus and Acipenser gueldenstaedtii. To identify mitotic changes during cell division, the nuclei of embryo blastomeres were sampled every 30 min from 4 to 25 h post-fertilization and stained with 5 mg/ml 4'-6-diaminido-2phenylindole. We observed embryos of hybrids to lose synchrony after the eighth cell division, one cycle earlier than in purebred sterlet embryos. To reveal the timing of zygotic gene activation, developing embryos were dechorionated and injected with 10, 50, 100, 250, or 500 mu g/ml a-Amanitin. Termination of development in embryos was recorded throughout the period from the 1000 cell stage until gastrulation, showing the process of transition from maternal to zygotic gene transcription in sterlet to be of extended duration and not directly linked to mid-blastula transition. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2020 Electronic address http://trjfas.org/abstract.php?id=1328
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