Study of 2-Nitrofluorene Interaction with DNA at a Glassy Carbon Electrode
1.
SYSNO ASEP
0459775
Document Type
C - Proceedings Paper (int. conf.)
R&D Document Type
Conference Paper
Title
Study of 2-Nitrofluorene Interaction with DNA at a Glassy Carbon Electrode
Author(s)
Skalová, Štěpánka (UFCH-W) Stávková, K. (CZ) Vyskočil, V. (CZ) Barek, J. (CZ)
Source Title
XXXVI. Moderní elektrochemické metody. Sborník přednášek. - Ústí nad Labem : Best servis, 2016 / Navrátil T. ; Fojta M. ; Schwarzová K.
- ISBN 978-80-905221-4-5
Pages
s. 196-200
Number of pages
5 s.
Publication form
Print - P
Action
Moderní elektrochemické metody /36./
Event date
23.05.2016 - 27.05.2016
VEvent location
Jetřichovice
Country
CZ - Czech Republic
Event type
WRD
Language
eng - English
Country
CZ - Czech Republic
Keywords
DNA biosensor ; glassy carbon electrode ; voltammetry
Subject RIV
CG - Electrochemistry
R&D Projects
GBP206/12/G151 GA ČR - Czech Science Foundation (CSF)
Institutional support
UFCH-W - RVO:61388955
UT WOS
000389898200045
Annotation
2-Nitrofluorene (2-NF) is a nitrated polycyclic aromatic hydrocarbon (NPAH) which occurs as the environmental pollutant. It is a potential carcinogen and mutagen. Interaction of deoxyribonucleic acid (DNA) with 2-NF was monitored using an electrochemical DNA biosensor prepared from a glassy carbon electrode (GCE) and low-molecular-weight DNA by electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), and square-wave voltammetry (SWV). There were no damaging interactions observed between DNA and 2-NF using EIS. However, CV shows intercalation of this substance into the DNA structure to form the complex 2-NF–DNA. Intercalation was also observed by SWV, confirming intercalation to reduce the number of electroactive sites and thus reducing the peak heights of adenosine and guanosine.
