Number of the records: 1

The dynamics of cytokinin changes after grafting of vegetative apices on flowering rapeseed plants

SYSNO ASEP	0504808
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	The dynamics of cytokinin changes after grafting of vegetative apices on flowering rapeseed plants
Author(s)	Tarkowská, Danuše (UEB-Q)_{RID, ORCID} Filek, M. (PL) Krekule, Jan (UEB-Q) Biesaga-Kościelniak, J. (PL) Marciñska, I. (PL) Popielarska-Konieczna, M. (PL) Strnad, Miroslav (UEB-Q)_{RID, ORCID}
Number of authors	7
Article number	78
Source Title	Plants. - : MDPI Roč. 8, č. 4 (2019)
Number of pages	8 s.
Language	eng - English
Country	CH - Switzerland
Keywords	Brassica napus ; Cytokinins ; Grafting ; Reproductive development ; Shoot apical meristem
Subject RIV	EF - Botanics
OECD category	Biochemical research methods
R&D Projects	EF16_019/0000738 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Open access
Institutional support	UEB-Q - RVO:61389030
UT WOS	000467296200001
EID SCOPUS	85064164861
DOI	10.3390/plants8040078
Annotation	Despite numerous studies, the role of hormones in the induction of shoot apical meristem leading to reproductive development, especially regarding thermoperiodic plants, is still not fully understood. The key problem is separating the effects of the low temperature required for vernalization from those responsible for low temperature stress. An earlier experiment demonstrated the correlation between an increase of cytokinin level in the apical parts of winter rapeseed and the transition time into their reproductive phase during vernalization, i.e., low temperature treatment. From data obtained from the presented experiments, this study aims to contribute to the understanding the role of cytokinins in the induction of flowering based on the grafting of vegetative apical parts of winter rapeseed (scion) on the reproductive (stock) winter and spring genotypes. On the basis of analyses carried out using ultra-high-performance liquid chromatography coupled with tandem mass spectrometry in combination with microscopic observation of changes at the apical meristem, it was indicated that the increase in the amount of trans-zeatin and trans- and cis-zeatin-O-glucoside derivatives appeared in the early stages of apex floral differentiation. During further development, the content of all investigated cytokinins passed through the maximum level followed by their decrease. The final level in reproductive apices was found to be higher than that in vegetative ones.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2020
Electronic address	http://doi.org/10.3390/plants8040078

Number of the records: 1