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Na+, K+/H+ antiporters regulate the pH of endoplasmic reticulum and auxin-mediated development

SYSNO ASEP	0489240
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Na+, K+/H+ antiporters regulate the pH of endoplasmic reticulum and auxin-mediated development
Author(s)	Fan, L. (CA) Zhao, L. (CN) Hu, W. (CN) Li, W. (CN) Novák, Ondřej (UEB-Q)_{RID, ORCID, SAI} Strnad, Miroslav (UEB-Q)_{RID, ORCID} Simon, S. (DE) Friml, J. (DE) Shen, J. (US) Jiang, L. (CN) Qiu, Q. S. (CN)
Number of authors	11
Source Title	Plant Cell and Environment. - : Wiley - ISSN 0140-7791 Roč. 41, č. 4 (2018), s. 850-864
Number of pages	15 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	Arabidopsis ; AtNHX5 ; AtNHX6 ; endosomal Na ,K /H antiporters ; pH ; pin5
Subject RIV	EB - Genetics ; Molecular Biology
OECD category	Cell biology
R&D Projects	LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	UEB-Q - RVO:61389030
UT WOS	000426870500012
EID SCOPUS	85042385885
DOI	10.1111/pce.13153
Annotation	AtNHX5 and AtNHX6 are endosomal Na + ,K + /H + antiporters that are critical for growth and development in Arabidopsis, but the mechanism behind their action remains unknown. Here, we report that AtNHX5 and AtNHX6, functioning as H + leak, control auxin homeostasis and auxin-mediated development. We found that nhx5 nhx6 exhibited growth variations of auxin-related defects. We further showed that nhx5 nhx6 was affected in auxin homeostasis. Genetic analysis showed that AtNHX5 and AtNHX6 were required for the function of the endoplasmic reticulum (ER)-localized auxin transporter PIN5. Although AtNHX5 and AtNHX6 were colocalized with PIN5 at ER, they did not interact directly. Instead, the conserved acidic residues in AtNHX5 and AtNHX6, which are essential for exchange activity, were required for PIN5 function. AtNHX5 and AtNHX6 regulated the pH in ER. Overall, AtNHX5 and AtNHX6 may regulate auxin transport across the ER via the pH gradient created by their transport activity. H +leak pathway provides a fine-tuning mechanism that controls cellular auxin fluxes.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2019

Number of the records: 1