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Short-term salt stress in Brassica rapa seedlings causes alterations in auxin metabolism

SYSNO ASEP	0489040
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Short-term salt stress in Brassica rapa seedlings causes alterations in auxin metabolism
Author(s)	Pavlović, I. (HR) Pěnčík, Aleš (UEB-Q)_{ORCID, RID, SAI} Novák, Ondřej (UEB-Q)_{RID, ORCID, SAI} Vujčić, V. (HR) Radić Brkanac, S. (HR) Lepeduš, H. (HR) Strnad, Miroslav (UEB-Q)_{RID, ORCID} Salopek-Sondi, B. (HR)
Number of authors	8
Source Title	Plant Physiology and Biochemistry. - : Elsevier - ISSN 0981-9428 Roč. 125, APR (2018), s. 74-84
Number of pages	11 s.
Language	eng - English
Country	FR - France
Keywords	Auxin metabolism ; Brassica rapa ssp. pekinensis ; Growth inhibition ; Principal component analysis ; Reactive oxygen species ; Short-term salinity stress ; Stress hormones
Subject RIV	EB - Genetics ; Molecular Biology
OECD category	Plant sciences, botany
R&D Projects	LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA17-06613S GA ČR - Czech Science Foundation (CSF)
Institutional support	UEB-Q - RVO:61389030
UT WOS	000428832200008
EID SCOPUS	85041546537
DOI	10.1016/j.plaphy.2018.01.026
Annotation	Salinity is one of major abiotic stresses affecting Brassica crop production. Here we present investigations into the physiological, biochemical, and hormonal components of the short-term salinity stress response in Chinese cabbage seedlings, with particular emphasis on the biosynthesis and metabolism of auxin indole-3-acetic acid (IAA). Upon salinity treatments (50–200 mM NaCl) IAA level was elevated in a dose dependent manner reaching 1.6-fold increase at the most severe salt treatment in comparison to the control. IAA precursor profiling suggested that salinity activated the indole-3-acetamide and indole-3-acetaldoxime biosynthetic pathways while suppressing the indole-3-pyruvic acid pathway. Levels of the IAA catabolites 2-oxoindole-3-acetic acid and indole-3-acetic acid-aspartate increased 1.7- and 2.0-fold, respectively, under the most severe treatment, in parallel with those of IAA. Conversely, levels of the ester conjugate indole-3-acetyl-1-O-ß-d-glucose and its catabolite 2-oxoindole-3-acetyl-1-O-ß-d-glucose decreased 2.5- and 7.0-fold, respectively. The concentrations of stress hormones including jasmonic acid and jasmonoyl-isoleucine (JA and JA-Ile), salicylic acid (SA) and abscisic acid (ABA) confirmed the stress induced by salt treatment: levels of JA and JA-Ile increased strongly under the mildest treatment, ABA only increased under the most severe treatment, and SA levels decreased dose-dependently. These hormonal changes were related to the observed changes in biochemical stress markers upon salt treatments: reductions in seedling fresh weight and root growth, decreased photosynthesis rate, increased levels of reactive oxygen species, and elevated proline content and the Na + /K + ratio. Correlations among auxin profile and biochemical stress markers were discussed based on Pearson's coefficients and principal component analysis (PCA).
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2019

Number of the records: 1