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Features of the organization of bread wheat chromosome 5BS based on physical mapping
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SYSNO ASEP 0488796 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Features of the organization of bread wheat chromosome 5BS based on physical mapping Author(s) Salina, E.A. (CU)
Nesterov, V. (RU)
Frenkel, Z. (IL)
Kiseleva, V. I. (RU)
Timonova, E. M. (RU)
Magni, F. (IT)
Vrána, Jan (UEB-Q) RID, ORCID
Šafář, Jan (UEB-Q) RID, ORCID
Šimková, Hana (UEB-Q) RID, ORCID
Doležel, Jaroslav (UEB-Q) RID, ORCID
Korol, A. (IL)
Sergeeva, E.M. (RU)Number of authors 12 Article number 80 Source Title BMC Genomics. - : BioMed Central - ISSN 1471-2164
Roč. 19, FEB 9 (2018)Number of pages 13 s. Language eng - English Country US - United States Keywords Chromosome 5BS ; Genetic markers ; Hexaploid wheat ; Physical mapping ; Sequencing ; Synteny ; Triticum aestivum Subject RIV EB - Genetics ; Molecular Biology OECD category Genetics and heredity (medical genetics to be 3) R&D Projects GBP501/12/G090 GA ČR - Czech Science Foundation (CSF) LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000424782900011 EID SCOPUS 85041808764 DOI 10.1186/s12864-018-4470-y Annotation Background: The IWGSC strategy for construction of the reference sequence of the bread wheat genome is based on first obtaining physical maps of the individual chromosomes. Our aim is to develop and use the physical map for analysis of the organization of the short arm of wheat chromosome 5B (5BS) which bears a number of agronomically important genes, including genes conferring resistance to fungal diseases. Results: A physical map of the 5BS arm (290 Mbp) was constructed using restriction fingerprinting and LTC software for contig assembly of 43,776 BAC clones. The resulting physical map covered ~ 99% of the 5BS chromosome arm (111 scaffolds, N50 = 3.078 Mb). SSR, ISBP and zipper markers were employed for anchoring the BAC clones, and from these 722 novel markers were developed based on previously obtained data from partial sequencing of 5BS. The markers were mapped using a set of Chinese Spring (CS) deletion lines, and F2 and RICL populations from a cross of CS and CS-5B dicoccoides. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2019
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