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Antibody-mediated modulation of cytokinins in tobacco: Organ-specific changes in cytokinin homeostasis
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SYSNO ASEP 0488685 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Antibody-mediated modulation of cytokinins in tobacco: Organ-specific changes in cytokinin homeostasis Author(s) Gelová, Z. (CZ)
Hoopen, P. (GB)
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Motyka, Václav (UEB-Q) RID, ORCID
Pernisová, M. (CZ)
Dabravolski, S. (CZ)
Didi, V. (CZ)
Tillack, F. (DE)
Oklešťková, Jana (UEB-Q) RID, ORCID, SAI
Strnad, Miroslav (UEB-Q) RID, ORCID
Hause, B. (DE)
Haruštiaková, D. (CZ)
Conrad, U. (DE)
Janda, L. (CZ)
Hejátko, J. (CZ)Number of authors 15 Source Title Journal of Experimental Botany. - : Oxford University Press - ISSN 0022-0957
Roč. 69, č. 3 (2018), s. 441-454Number of pages 14 s. Language eng - English Country GB - United Kingdom Keywords Antibody-mediated modulation ; biosynthesis ; ckx ; cytokinin ; homeostasis ; organ specificity ; tobacco Subject RIV EF - Botanics OECD category Plant sciences, botany R&D Projects LQ1601 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA16-14649S GA ČR - Czech Science Foundation (CSF) Institutional support UEB-Q - RVO:61389030 UT WOS 000423706500009 EID SCOPUS 85041217592 DOI 10.1093/jxb/erx426 Annotation Cytokinins comprise a group of phytohormones with an organ-specific mode of action. Although the mechanisms controlling the complex networks of cytokinin metabolism are partially known, the role of individual cytokinin types in the maintenance of cytokinin homeostasis remains unclear. Utilizing the overproduction of single-chain Fv antibodies selected for their ability to bind trans-zeatin riboside and targeted to the endoplasmic reticulum, we post-synthetically modulated cytokinin ribosides, the proposed transport forms of cytokinins. We observed asymmetric activity of cytokinin biosynthetic genes and cytokinin distribution in wild-Type tobacco seedlings with higher cytokinin abundance in the root than in the shoot. Antibody-mediated modulation of cytokinin ribosides further enhanced the relative cytokinin abundance in the roots and induced cytokinin-related phenotypes in an organ-specific manner. The activity of cytokinin oxidase/dehydrogenase in the roots was strongly up-regulated in response to antibody-mediated formation of the cytokinin pool in the endoplasmic reticulum. However, we only detected a slight decrease in the root cytokinin levels. In contrast, a significant decrease of cytokinins occurred in the shoot. We suggest the roots as the main site of cytokinin biosynthesis in tobacco seedlings. Conversely, cytokinin levels in the shoot seem to depend largely on long-range transport of cytokinin ribosides from the root and their subsequent metabolic activation. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2019
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