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Heterodera schachtii Tyrosinase-like protein a novel nematode effector modulating plant hormone homeostasis
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SYSNO ASEP 0488190 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Heterodera schachtii Tyrosinase-like protein a novel nematode effector modulating plant hormone homeostasis Author(s) Habash, S. (DE)
Radakovic, Z.S. (DE)
Vaňková, Radomíra (UEB-Q) RID, ORCID
Siddique, S. (DE)
Dobrev, Petre (UEB-Q) RID, ORCID
Gleason, C. (US)
Grundler, F.M.W. (DE)
Elashry, A. (DE)Number of authors 8 Article number 6874 Source Title Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 7, JUL 31 (2017)Number of pages 10 s. Language eng - English Country GB - United Kingdom Keywords arabidopsis-thaliana ; cyst-nematode ; parasitic nematode ; transient expression ; host plants ; sequence ; identification ; infection ; model ; transformation Subject RIV ED - Physiology OECD category Plant sciences, botany Institutional support UEB-Q - RVO:61389030 UT WOS 000425969300007 DOI 10.1038/s41598-017-07269-7 Annotation The beet cyst nematode Heterodera schachtii causes major yield losses in sugar beet. Understanding the interaction between H. schachtii and its host plant is important for developing a sustainable management system. Nematode effectors play a crucial role in initializing and sustaining successful parasitism. In our study, we identified a gene (Hs-Tyr) encoding a tyrosinase functional domain (PF00264). We describe Hs-Tyr as a novel nematode effector. Hs-Tyr is localized in the nematode esophageal gland. Up-regulation of its expression coincided with the parasitic developmental stages of the nematode. Silencing Hs-Tyr by RNA interference made the treated nematodes less virulent. When RNAi-treated nematodes succeeded in infecting the plant, developing females and their associated syncytial nurse cells were significantly smaller than in control plants. Ectopically expressing the Hs-Tyr effector in Arabidopsis increased plant susceptibility to H. schachtii, but not to the root-knot nematode Meloidogyne incognita. Interestingly, Hs-Tyr in the plant promoted plant growth and changed the root architecture. Additionally, the expression of Hs-Tyr in Arabidopsis caused changes in the homeostasis of several plant hormones especially auxin and the ethylene precursor aminocyclopropane-carboxylic acid. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2018
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