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ZNF143 protein is an important regulator of the myeloid transcription factor C/EBP
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SYSNO ASEP 0486300 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title ZNF143 protein is an important regulator of the myeloid transcription factor C/EBP Author(s) Gonzalez, D. (SG)
Luyten, A. (SG)
Bartholdy, B. (US)
Zhou, Q. (SG)
Kardošová, Miroslava (UMG-J)
Ebralidze, A. (US)
Swanson, K.D. (US)
Radomska, H.S. (US)
Zhang, P. (US)
Kobayashi, S.S. (US)
Welner, R.S. (US)
Levantini, E. (US)
Steidl, U. (US)
Chong, G. (SG)
Collombet, S. (SG)
Choi, M.H. (SG)
Friedman, A.D. (US)
Scott, L.M. (AU)
Alberich-Jorda, Meritxell (UMG-J) RID
Tenen, D.G. (US)Number of authors 20 Source Title Journal of Biological Chemistry. - : Elsevier - ISSN 0021-9258
Roč. 292, č. 46 (2017), s. 18924-18936Number of pages 13 s. Language eng - English Country US - United States Keywords CCAAT-enhancer-binding protein ; gene regulation ; hematopoiesis ; promoter ; transcription factor ; EBPalpha ; ZNF143 Subject RIV EB - Genetics ; Molecular Biology OECD category Cell biology Institutional support UMG-J - RVO:68378050 UT WOS 000415848000017 DOI 10.1074/jbc.M117.811109 Annotation The transcription factor C/EBP is essential for myeloid differentiation and is frequently dysregulated in acute myeloid leukemia. Although studied extensively, the precise regulation of its gene by upstream factors has remained largely elusive. Here, we investigated its transcriptional activation during myeloid differentiation. We identified an evolutionarily conserved octameric sequence, CCCAGCAG, approximate to 100 bases upstream of the CEBPA transcription start site, and demonstrated through mutational analysis that this sequence is crucial for C/EBP expression. This sequence is present in the genes encoding C/EBP in humans, rodents, chickens, and frogs and is also present in the promoters of other C/EBP family members. We identified that ZNF143, the human homolog of the Xenopus transcriptional activator STAF, specifically binds to this 8-bp sequence to activate C/EBP expression in myeloid cells through a mechanism that is distinct from that observed in liver cells and adipocytes. Altogether, our data suggest that ZNF143 plays an important role in the expression of C/EBP in myeloid cells. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2018
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