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Click chemistry-based tracking reveals putative cell wall-located auxin binding sites in expanding cells
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SYSNO ASEP 0485861 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Click chemistry-based tracking reveals putative cell wall-located auxin binding sites in expanding cells Author(s) Mravec, J. (DE)
Kračun, S. K. (DK)
Zemlyanskaya, E. (CZ)
Rydahl, M. G. (DK)
Guo, X. (DK)
Pičmanová, M. (DK)
Sørensen, K. (DK)
Růžička, Kamil (UEB-Q) ORCID
Willats, W.G.T. (DK)Number of authors 9 Article number 15988 Source Title Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 7, NOV 22 (2017)Number of pages 13 s. Language eng - English Country GB - United Kingdom Keywords MEMBRANE H+-ATPASE ; BIOLOGICAL-ACTIVITY ; AZIDO AUXINS Subject RIV EB - Genetics ; Molecular Biology OECD category Cell biology R&D Projects LQ1601 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000416118300012 EID SCOPUS 85034852105 DOI 10.1038/s41598-017-16281-w Annotation Auxin is a key plant regulatory molecule, which acts upon a plethora of cellular processes, including those related to cell differentiation and elongation. Despite the stunning progress in all disciplines of auxin research, the mechanisms of auxin-mediated rapid promotion of cell expansion and underlying rearrangement of cell wall components are poorly understood. This is partly due to the limitations of current methodologies for probing auxin. Here we describe a click chemistry-based approach, using an azido derivative of indole-3-propionic acid. This compound is as an active auxin analogue, which can be tagged in situ. Using this new tool, we demonstrate the existence of putative auxin binding sites in the cell walls of expanding/elongating cells. These binding sites are of protein nature but are distinct from those provided by the extensively studied AUXIN BINDING PROTEIN 1 (ABP1). Using immunohistochemistry, we have shown the apoplastic presence of endogenous auxin epitopes recognised by an anti-IAA antibody. Our results are intriguingly in line with previous observations suggesting some transcription-independent (non-genomic) activity of auxin in cell elongation. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2018
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