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Regulation of the membrane structure by brassinosteroids and progesterone in winter wheat seedlings exposed to low temperature
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SYSNO ASEP 0485772 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Regulation of the membrane structure by brassinosteroids and progesterone in winter wheat seedlings exposed to low temperature Author(s) Filek, M. (PL)
Rudolphi-Skórska, E. (PL)
Sieprawska, A. (PL)
Kvasnica, Miroslav (UEB-Q) RID, ORCID
Janeczko, A. (PL)Number of authors 5 Source Title Steroids. - : Elsevier - ISSN 0039-128X
Roč. 128, DEC (2017), s. 37-45Number of pages 9 s. Language eng - English Country US - United States Keywords 24-Epibrassinolide ; 24-Epicastasterone ; Galactolipids ; Phospholipids ; Progesterone ; Seedlings ; Winter wheat Subject RIV CC - Organic Chemistry OECD category Organic chemistry R&D Projects GJ15-08202Y GA ČR - Czech Science Foundation (CSF) Institutional support UEB-Q - RVO:61389030 UT WOS 000418216100006 EID SCOPUS 85032271899 DOI 10.1016/j.steroids.2017.10.002 Annotation Steroids constitute one of the most important groups of compounds of regulatory properties both in the animal and plant kingdom. In plants, steroids such as brassinosteroids or progesterone, by binding to protein receptors in cell membranes, regulate growth and initiate processes leading to increased tolerance to stress conditions. Due to their structural similarities to sterols, these steroids may also directly interact with cellular membranes. Our aim was to determine the changes of the structural parameters of lipid membranes under the influence of hydrophobic steroid compounds, i.e., 24-epibrassinolide (EBR) and its precursor—24-epicastasterone (ECS) and progesterone (PRO). Lipids were isolated from wheat seedlings with different tolerances to frost, grown at low temperatures (5 °C) for 1.5 and 3 weeks (acclimation process). Control plants were cultured continuously at 20 °C. From galactolipids and phospholipids, the main polar lipid fractions, the monolayers were formed, using a technique of Langmuir trough. EBR and ECS were introduced into monolayers, together with lipids, whereas the PRO was dissolved in the aqueous sub-phase upon which the monolayers were spread. Measurements performed at 25 °C and 10 °C showed a significant action of th e tested compounds on the physicochemical properties of the monolayers. EBR and PRO increased the area per lipid molecule in monolayers, resulting in formation of more flexible surface structures while the presence of the ECS induced the opposite effect. The influence of the polarity of lipids and steroids on the interactions in the monolayer was discussed. Lipids extracted from the membranes of wheat with the most tolerance to frost were characterized by the highest fatty acid unsaturation and steroids had a relatively weak effect on the parameters of the structure of their monolayers. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2018
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