Number of the records: 1

Physical map of the short arm of bread wheat chromosome 3D

SYSNO ASEP	0479190
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Physical map of the short arm of bread wheat chromosome 3D
Author(s)	Holušová, Kateřina (UEB-Q)_ORCID Vrána, Jan (UEB-Q)_{RID, ORCID} Šafář, Jan (UEB-Q)_{RID, ORCID} Šimková, Hana (UEB-Q)_{RID, ORCID} Balcárková, Barbora (UEB-Q) Frenkel, Z. (IL) Darrier, B. (FR) Paux, E. (FR) Cattonaro, F. (IT) Berges, H. (FR) Letellier, T. (FR) Alaux, M. (FR) Doležel, Jaroslav (UEB-Q)_{RID, ORCID} Bartoš, Jan (UEB-Q)_{RID, ORCID}
Number of authors	14
Source Title	Plant Genome. - : Wiley Roč. 10, č. 2 (2017)
Number of pages	11 s.
Language	eng - English
Country	US - United States
Keywords	TRITICUM-AESTIVUM L. ; COMPLEX GENOMES ; INDIVIDUAL CHROMOSOMES
Subject RIV	EB - Genetics ; Molecular Biology
OECD category	Plant sciences, botany
R&D Projects	GA13-08786S GA ČR - Czech Science Foundation (CSF)
	LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	UEB-Q - RVO:61389030
UT WOS	000410819500025
EID SCOPUS	85024474147
DOI	10.3835/plantgenome2017.03.0021
Annotation	Bread wheat (Triticum aestivum L.) is one of the most important crops worldwide. Although a reference genome sequence would represent a valuable resource for wheat improvement through genomics-assisted breeding and gene cloning, its generation has long been hampered by its allohexaploidy, high repeat content, and large size. As a part of a project coordinated by the International Wheat Genome Sequencing Consortium (IWGSC), a physical map of the short arm of wheat chromosome 3D (3DS) was prepared to facilitate reference genome assembly and positional gene cloning. It comprises 869 contigs with a cumulative length of 274.5 Mbp and represents 85.5% of the estimated chromosome arm size. Eighty-six Mbp of survey sequences from chromosome arm 3DS were assigned in silico to physical map contigs via next-generation sequencing of bacterial artificial chromosome pools, thus providing a high-density framework for physical map ordering along the chromosome arm. About 60% of the physical map was anchored in this single experiment. Finally, 1393 high-confidence genes were anchored to the physical map. Comparisons of gene space of the chromosome arm 3DS with genomes of closely related species [Brachypodium distachyon (L.) P.Beauv., rice (Oryza sativa L.), and sorghum [Sorghum bicolor (L.) Moench] and homeologous wheat chromosomes provided information about gene movement on the chromosome arm.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2018

Number of the records: 1