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ACCERBATIN, a small molecule at the intersection of auxin and reactive oxygen species homeostasis with herbicidal properties
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SYSNO ASEP 0479102 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title ACCERBATIN, a small molecule at the intersection of auxin and reactive oxygen species homeostasis with herbicidal properties Author(s) Hu, Y. (US)
Depaepe, T. (BE)
Smet, D. (BE)
Hoyerová, Klára (UEB-Q) RID, ORCID
Klíma, Petr (UEB-Q) RID, ORCID
Cuypers, J. (BE)
Cutler, S. (GB)
Buyst, D. (BE)
Morreel, K. (BE)
Boerjan, W. (BE)
Martins, J. (BE)
Petrášek, Jan (UEB-Q) RID, ORCID
Vandenbussche, F. (BE)
Van Der Straeten, D. (BE)Number of authors 14 Source Title Journal of Experimental Botany. - : Oxford University Press - ISSN 0022-0957
Roč. 68, č. 15 (2017), s. 4185-4203Number of pages 19 s. Language eng - English Country GB - United Kingdom Keywords apical hook development ; root hair development ; arabidopsis-thaliana seedlings ; ethylene biosynthesis ; shoot gravitropism ; cell elongation ; abiotic stress ; abscisic-acid ; plant-growth ; gene family ; Arabidopsis ; auxin homeostasis ; chemical genetics ; ethylene signaling ; herbicide ; quinoline carboxamide ; reactive oxygen species ; triple response Subject RIV EA - Cell Biology OECD category Plant sciences, botany R&D Projects LD15137 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000410245700017 DOI 10.1093/jxb/erx242 Annotation The volatile two-carbon hormone ethylene acts in concert with an array of signals to affect etiolated seedling development. From a chemical screen, we isolated a quinoline carboxamide designated ACCERBATIN (AEX) that exacerbates the 1-aminocyclopropane-1-carboxylic acid-induced triple response, typical for ethylene-treated seedlings in darkness. Phenotypic analyses revealed distinct AEX effects including inhibition of root hair development and shortening of the root meristem. Mutant analysis and reporter studies further suggested that AEX most probably acts in parallel to ethylene signaling. We demonstrated that AEX functions at the intersection of auxin metabolism and reactive oxygen species (ROS) homeostasis. AEX inhibited auxin efflux in BY-2 cells and promoted indole-3-acetic acid (IAA) oxidation in the shoot apical meristem and cotyledons of etiolated seedlings. Gene expression studies and superoxide/hydrogen peroxide staining further revealed that the disrupted auxin homeostasis was accompanied by oxidative stress. Interestingly, in light conditions, AEX exhibited properties reminiscent of the quinoline carboxylate-type auxin-like herbicides. We propose that AEX interferes with auxin transport from its major biosynthesis sites, either as a direct consequence of poor basipetal transport from the shoot meristematic region, or indirectly, through excessive IAA oxidation and ROS accumulation. Further investigation of AEX can provide new insights into the mechanisms connecting auxin and ROS homeostasis in plant development and provide useful tools to study auxin-type herbicides. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2018
Number of the records: 1