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Inheritance patterns of the response to in vitro doubled haploid induction in perennial ryegrass (Lolium perenne L.)
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SYSNO ASEP 0478745 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Inheritance patterns of the response to in vitro doubled haploid induction in perennial ryegrass (Lolium perenne L.) Author(s) Begheyn, R. F. (CH)
Roulund, N. (DK)
Vangsgaard, K. (DK)
Kopecký, David (UEB-Q) RID, ORCID
Studer, B. (CH)Number of authors 5 Source Title Plant Cell, Tissue and Organ Culture. - : Springer - ISSN 0167-6857
Roč. 130, č. 3 (2017), s. 667-679Number of pages 13 s. Language eng - English Country NL - Netherlands Keywords Androgenesis ; Androgenic capacity ; Anther culture ; Doubled haploid (DH) ; Perennial ryegrass (Lolium perenne L.) Subject RIV EB - Genetics ; Molecular Biology OECD category Plant sciences, botany Institutional support UEB-Q - RVO:61389030 UT WOS 000408008200018 EID SCOPUS 85021061151 DOI 10.1007/s11240-017-1255-y Annotation The ability to produce doubled haploid (DH) plants has found broad application in research and breeding. For major crop species such as maize (Zea mays L.) and barley (Hordeum vulgare L.), routine large-scale p roduction of DHs has enabled the acceleration of breeding processes, for example through efficient generation of homozygous lines. However, in forage crops such as perennial ryegrass (Lolium perenne L.), low and genotype-specific responses to in vitro anther culture (AC) still limit wide-spread use of DHs. Here, we report the responses of nine bi-parental populations, segregating for microspore embryogenesis and plant regeneration capacity, to an effective AC protocol. Genotypes of exceptionally high androgenic ability, producing over 200 green plants per 100 anthers cultured, could be selected. Continuous and distinctly shaped distributions for the evaluated traits were indicative of quantitative polygenic control and the presence of different alleles in each population. An insignificant association of embryo production with plant regeneration, as well as a low correlation between green and albino plant yield (ρ = 0.20), suggested that different genes influence these traits. The populations evaluated here provide a rich source of alleles needed for the introgression of high levels of androgenic capacity into recalcitrant material. Moreover, this germplasm is ideally suited for use in future genotyping and mapping studies so that the genetic control of androgenic capacity in perennial ryegrass can be elucidated. Ultimately, our results will help to realize the potential of DH induction in one of the world’s most important forage crop species. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2018
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