Number of the records: 1

Brassinosteroid biosynthesis is modulated via a transcription factor cascade of COG1, PIF4, and PIF5

SYSNO ASEP	0476568
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Brassinosteroid biosynthesis is modulated via a transcription factor cascade of COG1, PIF4, and PIF5
Author(s)	Wei, Z. (CN) Yuan, T. (US) Tarkowská, Danuše (UEB-Q)_{RID, ORCID} Kim, J. (KR) Nam, H. G. (KR) Novák, Ondřej (UEB-Q)_{RID, ORCID, SAI} He, K. (CN) Gou, X. (CN) Li, J. (CN)
Number of authors	9
Source Title	Plant Physiology. - : Oxford University Press - ISSN 0032-0889 Roč. 174, č. 2 (2017), s. 1260-1273
Number of pages	14 s.
Language	eng - English
Country	US - United States
Keywords	GSK3-LIKE KINASE BIN2 ; ARABIDOPSIS-THALIANA ; CATHARANTHUS-ROSEUS
Subject RIV	EF - Botanics
OECD category	Plant sciences, botany
R&D Projects	LK21306 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA14-34792S GA ČR - Czech Science Foundation (CSF)
Institutional support	UEB-Q - RVO:61389030
UT WOS	000403152200064
EID SCOPUS	85020482386
DOI	10.1104/pp.16.01778
Annotation	Brassinosteroids (BRs) are essential phytohormones regulating various developmental and physiological processes during normal growth and development. cog1-3D (cogwheel1-3D) was identified as an activation-tagged genetic modifier of bri1-5, an intermediate BR receptor mutant in Arabidopsis (Arabidopsis thaliana). COG1 encodes a Dof-type transcription factor found previously to act as a negative regulator of the phytochrome signaling pathway. cog1-3D single mutants show an elongated hypocotyl phenotype under light conditions. A loss-of-function mutant or inducible expression of a dominant negative form of COG1 in the wild type results in an opposite phenotype. A BR profile assay indicated that BR levels are elevated in cog1-3D seedlings. Quantitative reverse transcription-polymerase chain reaction analyses showed that several key BR biosynthetic genes are significantly up-regulated in cog1-3D compared with those of the wild type. Two basic helix-loop-helix transcription factors, PIF4 and PIF5, were found to be transcriptionally up-regulated in cog1-3D. Genetic analysis indicated that PIF4 and PIF5 were required for COG1 to promote BR biosynthesis and hypocotyl elongation. Chromatin immunoprecipitation and electrophoretic mobility shift assays indicated that COG1 binds to the promoter regions of PIF4 and PIF5, and PIF4 and PIF5 bind to the promoter regions of key BR biosynthetic genes, such as DWF4 and BR6ox2, to directly promote their expression. These results demonstrated that COG1 regulates BR biosynthesis via up-regulating the transcription of PIF4 and PIF5.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2018

Number of the records: 1