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Exploiting repetitive sequences and BAC clones in Festuca pratensis karyotyping
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SYSNO ASEP 0476566 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Exploiting repetitive sequences and BAC clones in Festuca pratensis karyotyping Author(s) Majka, J. (PL)
Ksiazczyk, T. (PL)
Kiełbowicz-Matuk, A. (PL)
Kopecký, David (UEB-Q) RID, ORCID
Kosmala, A. (PL)Number of authors 5 Article number e0179043 Source Title PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 12, č. 6 (2017)Number of pages 15 s. Language eng - English Country US - United States Keywords IN-SITU HYBRIDIZATION ; RIBOSOMAL-RNA GENES ; LOLIUM-MULTIFLORUM Subject RIV EB - Genetics ; Molecular Biology OECD category Plant sciences, botany Institutional support UEB-Q - RVO:61389030 UT WOS 000402880700078 EID SCOPUS 85020455160 DOI 10.1371/journal.pone.0179043 Annotation The Festuca genus is thought to be the most numerous genus of the Poaceae family. One of the most agronomically important forage grasses, Festuca pratensis Huds. is treated as a model plant to study the molecular mechanisms associated with tolerance to winter stresses, including frost. However, the precise mapping of the genes governing stress tolerance in this species is difficult as its karyotype remains unrecognized. Only two F. pratensis chromosomes with 35S and 5S rDNA sequences can be easily identified, but its remaining chromosomes have not been distinguished to date. Here, two libraries derived from F. pratensis nuclear DNA with various contents of repetitive DNA sequences were used as sources of molecular probes for fluorescent in situ hybridisation (FISH), a BAC library and a library representing sequences most frequently present in the F. pratensis genome. Using FISH, six groups of DNA sequences were revealed in chromosomes on the basis of their signal position, including dispersed-like sequences, chromosome painting-like sequences, centromeric-like sequences, knob-like sequences, a group without hybridization signals, and single locus-like sequences. The last group was exploited to develop cytogenetic maps of diploid and tetraploid F. pratensis, which are presented here for the first time and provide a remarkable progress in karyotype characterization. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2018
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