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Infection by rhodococcus fascians maintains cotyledons as a sink tissue for the pathogen

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    SYSNO ASEP0476550
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleInfection by rhodococcus fascians maintains cotyledons as a sink tissue for the pathogen
    Author(s) Dhandapani, P. (NZ)
    Song, J. (US)
    Novák, Ondřej (UEB-Q) RID, ORCID, SAI
    Jameson, P. E. (NZ)
    Number of authors4
    Source TitleAnnals of Botany - ISSN 0305-7364
    Roč. 119, č. 5 (2017), s. 841-852
    Number of pages12 s.
    Languageeng - English
    CountryGB - United Kingdom
    KeywordsAmino acid transporter ; Apical dominance ; Cell wall invertase ; Cytokinin ; Cytokinin oxidase/dehydro-genase ; Pea ; Pisum sativum L. ; Rhodococcus fascians ; Seed ; Sink and source ; Sucrose transporter ; sweet
    Subject RIVEB - Genetics ; Molecular Biology
    OBOR OECDPlant sciences, botany
    R&D ProjectsLO1204 GA MŠk - Ministry of Education, Youth and Sports (MEYS)
    LK21306 GA MŠk - Ministry of Education, Youth and Sports (MEYS)
    Institutional supportUEB-Q - RVO:61389030
    UT WOS000400982600013
    EID SCOPUS85018991925
    DOI10.1093/aob/mcw202
    AnnotationBackground and Aims: Pisum sativum L. (pea) seed is a source of carbohydrate and protein for the developing plant. By studying pea seeds inoculated by the cytokinin-producing bacterium, Rhodococcus fascians, we sought to determine the impact of both an epiphytic (avirulent) strain and a pathogenic strain on source-sink activity within the cotyledons during and following germination.
    Methods: Bacterial spread was monitored microscopically, and real-time reverse transcription-quantitative PCR was used to determine the expression of cytokinin biosynthesis, degradation and response regulator gene family members, along with expression of family members of SWEET, SUT, CWINV and AAP genes gene families identified initially in pea by transcriptomic analysis. The endogenous cytokinin content was also determined.
    Key Results: The cotyledons infected by the virulent strain remained intact and turned green, while multiple shoots were formed and root growth was reduced. The epiphytic strain had no such marked impact. Isopentenyl adenine was elevated in the cotyledon s infected by the virulent strain. Strong expression of RfIPT, RfLOG and RfCKX was detected in the cotyledons infected by the virulent strain throughout the experiment, with elevated expression also observed for PsSWEET, PsSUT and PsINV gene family members. The epiphytic strain had some impact on the expression of these genes, especially at the later stages of reserve mobilization from the cotyledons.
    Conclusions: The pathogenic strain retained the cotyledons as a sink tissue for the pathogen rather than the cotyledon converting completely to a source tissue for the germinating plant. We suggest that the interaction of cytoki-nins, CWINVs and SWEETs may lead to the loss of apical dominance and the appearance of multiple shoots.
    WorkplaceInstitute of Experimental Botany
    ContactDavid Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
    Year of Publishing2018
Number of the records: 1