Number of the records: 1

Infection by rhodococcus fascians maintains cotyledons as a sink tissue for the pathogen

SYSNO ASEP	0476550
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Infection by rhodococcus fascians maintains cotyledons as a sink tissue for the pathogen
Author(s)	Dhandapani, P. (NZ) Song, J. (US) Novák, Ondřej (UEB-Q)_{RID, ORCID, SAI} Jameson, P. E. (NZ)
Number of authors	4
Source Title	Annals of Botany. - : Oxford University Press - ISSN 0305-7364 Roč. 119, č. 5 (2017), s. 841-852
Number of pages	12 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	Amino acid transporter ; Apical dominance ; Cell wall invertase ; Cytokinin ; Cytokinin oxidase/dehydro-genase ; Pea ; Pisum sativum L. ; Rhodococcus fascians ; Seed ; Sink and source ; Sucrose transporter ; sweet
Subject RIV	EB - Genetics ; Molecular Biology
OECD category	Plant sciences, botany
R&D Projects	LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LK21306 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	UEB-Q - RVO:61389030
UT WOS	000400982600013
EID SCOPUS	85018991925
DOI	10.1093/aob/mcw202
Annotation	Background and Aims: Pisum sativum L. (pea) seed is a source of carbohydrate and protein for the developing plant. By studying pea seeds inoculated by the cytokinin-producing bacterium, Rhodococcus fascians, we sought to determine the impact of both an epiphytic (avirulent) strain and a pathogenic strain on source-sink activity within the cotyledons during and following germination. Methods: Bacterial spread was monitored microscopically, and real-time reverse transcription-quantitative PCR was used to determine the expression of cytokinin biosynthesis, degradation and response regulator gene family members, along with expression of family members of SWEET, SUT, CWINV and AAP genes gene families identified initially in pea by transcriptomic analysis. The endogenous cytokinin content was also determined. Key Results: The cotyledons infected by the virulent strain remained intact and turned green, while multiple shoots were formed and root growth was reduced. The epiphytic strain had no such marked impact. Isopentenyl adenine was elevated in the cotyledon s infected by the virulent strain. Strong expression of RfIPT, RfLOG and RfCKX was detected in the cotyledons infected by the virulent strain throughout the experiment, with elevated expression also observed for PsSWEET, PsSUT and PsINV gene family members. The epiphytic strain had some impact on the expression of these genes, especially at the later stages of reserve mobilization from the cotyledons. Conclusions: The pathogenic strain retained the cotyledons as a sink tissue for the pathogen rather than the cotyledon converting completely to a source tissue for the germinating plant. We suggest that the interaction of cytoki-nins, CWINVs and SWEETs may lead to the loss of apical dominance and the appearance of multiple shoots.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2018

Number of the records: 1