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Analysis of exocyst subunit EXO70 family reveals distinct membrane polar domains in Tobacco pollen tubes

SYSNO ASEP	0476519
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Analysis of exocyst subunit EXO70 family reveals distinct membrane polar domains in Tobacco pollen tubes
Author(s)	Sekereš, Juraj (UEB-Q)_{RID, ORCID} Pejchar, Přemysl (UEB-Q)_{RID, ORCID} Šantrůček, J. (CZ) Vukašinović, Nemanja (UEB-Q)_ORCID Žárský, Viktor (UEB-Q)_{RID, ORCID} Potocký, Martin (UEB-Q)_{RID, ORCID}
Number of authors	6
Source Title	Plant Physiology. - : Oxford University Press - ISSN 0032-0889 Roč. 173, č. 3 (2017), s. 1659-1675
Number of pages	17 s.
Language	eng - English
Country	US - United States
Keywords	PLASMA-MEMBRANE ; ARABIDOPSIS-THALIANA ; CELL-MIGRATION
Subject RIV	EB - Genetics ; Molecular Biology
OECD category	Plant sciences, botany
R&D Projects	GA13-19073S GA ČR - Czech Science Foundation (CSF)
	GA15-24711S GA ČR - Czech Science Foundation (CSF)
Method of publishing	Open access
Institutional support	UEB-Q - RVO:61389030
UT WOS	000400366900013
EID SCOPUS	85014614437
DOI	10.1104/pp.16.01709
Annotation	The vesicle-tethering complex exocyst is one of the crucial cell polarity regulators. The EXO70 subunit is required for the targeting of the complex and is represented by many isoforms in angiosperm plant cells. This diversity could be partly responsible for the establishment and maintenance of membrane domains with different composition. To address this hypothesis, we employed the growing pollen tube, a well-established cell polarity model system, and performed large-scale expression, localization, and functional analysis of tobacco (Nicotiana tabacum) EXO70 isoforms. Various isoforms localized to different regions of the pollen tube plasma membrane, apical vesicle-rich inverted cone region, nucleus, and cytoplasm. The overexpression of major pollen-expressed EXO70 isoforms resulted in growth arrest and characteristic phenotypic deviations of tip swelling and apical invaginations. NtEXO70A1a and NtEXO70B1 occupied two distinct and mutually exclusive plasma membrane domains. Both isoforms partly colocalized with the exocyst subunit NtSEC3a at the plasma membrane, possibly forming different exocyst complex subpopulations. NtEXO70A1a localized to the small area previously characterized as the site of exocytosis in the tobacco pollen tube, while NtEXO70B1 surprisingly colocalized with the zone of clathrin-mediated endocytosis. Both NtEXO70A1a and NtEXO70B1 colocalized to different degrees with markers for the anionic signaling phospholipids phosphatidylinositol 4,5-bisphosphate and phosphatidic acid. In contrast, members of the EXO70 C class, which are specifically expressed in tip-growing cells, exhibited exocytosis-related functional effects in pollen tubes despite the absence of apparent plasma membrane localization. Taken together, our data support the existence of multiple membranetrafficking domains regulated by different EXO70-containing exocyst complexes within a single cell.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2018
Electronic address	http://www.plantphysiol.org/content/173/3/1659

Number of the records: 1