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Effects of 24-epibrassinolide and green light on plastid gene transcription and cytokinin content of barley leaves
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SYSNO ASEP 0476453 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Effects of 24-epibrassinolide and green light on plastid gene transcription and cytokinin content of barley leaves Author(s) Efimova, N.V. (RU)
Vaňková, Radomíra (UEB-Q) RID, ORCID
Kusnetsov, V. (RU)
Litvinovskaya, R. P. (BY)
Zlobin, Y.L. (RU)
Dobrev, Petre (UEB-Q) RID, ORCID
Vedenicheva, N.P. (RU)
Savchuk, A. L. (RU)
Karnachuk, R. A. (RU)
Kudryakova, N.V. (RU)
Kuznetsov, V. D. (RU)Number of authors 11 Source Title Steroids. - : Elsevier - ISSN 0039-128X
Roč. 120, APR (2017), s. 32-40Number of pages 9 s. Language eng - English Country US - United States Keywords lupinus-luteus cotyledons ; arabidopsis-thaliana ; de-etiolation ; abscisic-acid ; brassinosteroid biosynthesis ; plant development ; hormonal balance ; mutant ; expression ; growth ; Brassinosteroids ; Cytokinin ; Gene expression ; Green light ; Hordeum vulgare ; Transcription Subject RIV EF - Botanics OECD category Plant sciences, botany Institutional support UEB-Q - RVO:61389030 UT WOS 000397687800005 DOI 10.1016/j.steroids.2016.12.004 Annotation In order to evaluate whether brassinosteroids (BS) and green light regulate the transcription of plastid genes in a cross-talk with cytokinins (CKs), transcription rates of 12 plastid genes (ndhF, rrn23, rpoB, psaA, psaB, rrn16, psbA, psbD, psbK, rbcL, atpB, and trnE/trnY) as well as the accumulation of transcripts of some photoreceptors (PHYA, CRY2, CRY1A, and CRY1B) and signaling (SERK and CAS) genes were followed in detached etiolated barley leaves exposed to darkness, green or white light +/- 1 mu m 24-epibrassinolide (EBL). EBL in the dark was shown to up-regulate the transcription of 12 plastid genes, while green light activated 10 genes and the EBL combined with the green light affected the transcription of only two genes (psaB and rpoB). Green light inhibited the expression of photoreceptor genes, except for CRY1A. Under the green light, EBL practically did not affect the expression of CRY1A, CAS and SERK genes, but it reduced the influence of white light on the accumulation of CAS, CRY1A, CRY1B, and SERK gene transcripts. The total content of BS in the dark and under white light remained largely unchanged, while under green light the total content of BRs (brassinolide, castasterone, and 6-deoxocastasterone) and HBR5 (28-homobrassinolide, 28-homocastasterone, and 6-deoxo-28-homocastasterone) increased. The EBL-dependent up-regulation of plastome transcription in the dark was accompanied by a significant decrease in CK deactivation by O-glucosylation. However, no significant effect on the content of active CKs was detected. EBL combined with green light moderately increased the contents of trans-zeatin and isopentenyladenine, but had a negative effect on cis-zeatin. The most significant promotive effect of EBL on active CK bases was observed in white light. The data obtained suggest the involvement of CKs in the BS-and light-dependent transcription regulation of plastid genes. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2018
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