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Early changes of the pH of the apoplast are different in leaves, stem and roots of Vicia faba L. under declining water availability
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SYSNO ASEP 0476350 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Early changes of the pH of the apoplast are different in leaves, stem and roots of Vicia faba L. under declining water availability Author(s) Karuppanapandian, T. (CZ)
Geilfus, C.M. (DE)
Muehling, K.H. (DE)
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Gloser, V. (CZ)Number of authors 5 Source Title Plant Science. - : Elsevier - ISSN 0168-9452
Roč. 255, FEB (2017), s. 51-58Number of pages 8 s. Language eng - English Country IE - Ireland Keywords xylem sap constituents ; abscisic-acid ; stomatal conductance ; leaf apoplast ; helianthus-annuus ; plant-responses ; intact plants ; nacl stress ; drying soil ; guard-cells ; Drought stress ; Abscisic acid ; Soil drying ; Xylem sap ; Osmolality ; Water relations ; Leaf water potential Subject RIV EF - Botanics OECD category Plant sciences, botany Institutional support UEB-Q - RVO:61389030 UT WOS 000394194800006 DOI 10.1016/j.plantsci.2016.11.010 Annotation Changes in pH of the apoplast have recently been discussed as an important factor in adjusting transpiration and water relations under conditions of drought via modulatory effect on abscisic acid (ABA) concentration. Using Vicia faba L., we investigated whether changes in the root, shoot and leaf apoplastic pH correlated with (1) a drought-induced reduction in transpiration and with (2) changes in ABA concentration. Transpiration, leaf water potential and ABA in leaves were measured and correlated with root and shoot xylem pH, determined by a pH microelectrode, and pH of leaf apoplast quantified by microscopy-based in vivo ratiometric analysis. Results revealed that a reduction in transpiration rate in the early phase of soil drying could not be linked with changes in the apoplastic pH via effects on the stomata-regulating hormone ABA. Moreover, drought-induced increase in pH of xylem or leaf apoplast was not the remote effect of an acropetal transport of alkaline sap from root, because root xylem acidified during progressive soil drying, whereas the shoot apoplast alkalized. We reason that other, yet unknown signalling mechanism was responsible for reduction of transpiration rate in the early phase of soil drying. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2018
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