Number of the records: 1

UNcleProt (Universal Nuclear Protein database of barley): The first nuclear protein database that distinguishes proteins from different phases of the cell cycle

SYSNO ASEP	0475907
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	UNcleProt (Universal Nuclear Protein database of barley): The first nuclear protein database that distinguishes proteins from different phases of the cell cycle
Author(s)	Blavet, Nicolas (UEB-Q)_{RID, ORCID} Uřinovská, J. (CZ) Jeřábková, Hana (UEB-Q)_{RID, ORCID} Chamrád, I. (CZ) Vrána, Jan (UEB-Q)_{RID, ORCID} Lenobel, R. (CZ) Beinhauer, D. (CZ) Šebela, M. (CZ) Doležel, Jaroslav (UEB-Q)_{RID, ORCID} Petrovská, Beáta (UEB-Q)_{RID, ORCID}
Number of authors	10
Source Title	Nucleus. - : Taylor & Francis - ISSN 1949-1034 Roč. 8, č. 1 (2017), s. 70-80
Number of pages	11 s.
Language	eng - English
Country	US - United States
Keywords	cicer-arietinum l. ; rice oryza-sativa ; chromatin-associated proteins ; proteomic analysis ; mitotic chromosomes ; dehydration ; localization ; chickpea ; network ; phosphoproteome ; barley ; cell cycle ; database ; flow-cytometry ; localization ; mass spectrometry ; nuclear proteome ; nucleus
Subject RIV	CE - Biochemistry
OECD category	Cell biology
R&D Projects	GA14-28443S GA ČR - Czech Science Foundation (CSF)
	LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	UEB-Q - RVO:61389030
UT WOS	000394948000008
DOI	10.1080/19491034.2016.1255391
Annotation	Proteins are the most abundant component of the cell nucleus, where they perform a plethora of functions, including the assembly of long DNA molecules into condensed chromatin, DNA replication and repair, regulation of gene expression, synthesis of RNA molecules and their modification. Proteins are important components of nuclear bodies and are involved in the maintenance of the nuclear architecture, transport across the nuclear envelope and cell division. Given their importance, the current poor knowledge of plant nuclear proteins and their dynamics during the cell's life and division is striking. Several factors hamper the analysis of the plant nuclear proteome, but the most critical seems to be the contamination of nuclei by cytosolic material during their isolation. With the availability of an efficient protocol for the purification of plant nuclei, based on flow cytometric sorting, contamination by cytoplasmic remnants can be minimized. Moreover, flow cytometry allows the separation of nuclei in different stages of the cell cycle (G1, S, and G2). This strategy has led to the identification of large number of nuclear proteins from barley (Hordeum vulgare), thus triggering the creation of a dedicated database called UNcleProt, http://barley.gambrinus.ueb.cas.cz/.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2018

Number of the records: 1