Number of the records: 1
Satellite DNA and Transposable Elements in Seabuckthorn (Hippophae rhamnoides), a Dioecious Plant with Small Y and Large X Chromosomes
- 1.
SYSNO ASEP 0475905 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Satellite DNA and Transposable Elements in Seabuckthorn (Hippophae rhamnoides), a Dioecious Plant with Small Y and Large X Chromosomes Author(s) Puterová, J. (CZ)
Razumova, O. (RU)
Martínek, T. (CZ)
Alexandrov, O. (RU)
Divashuk, M. (RU)
Kubát, Z. (CZ)
Hobza, Roman (UEB-Q) RID, ORCID
Karlov, G. (RU)
Kejnovský, E. (CZ)Number of authors 9 Source Title Genome Biology and Evolution. - : Oxford University Press - ISSN 1759-6653
Roč. 9, č. 1 (2017), s. 197-212Number of pages 16 s. Language eng - English Country GB - United Kingdom Keywords sex-chromosomes ; repetitive sequences ; silene-latifolia ; molecular cytogenetics ; arabidopsis-thaliana ; genome size ; evolution ; organization ; alignment ; database ; sex chromosomes ; genome composition ; chromosomal localization ; repetitive DNA Subject RIV EB - Genetics ; Molecular Biology OECD category Plant sciences, botany R&D Projects GBP501/12/G090 GA ČR - Czech Science Foundation (CSF) Institutional support UEB-Q - RVO:61389030 UT WOS 000396057400016 DOI 10.1093/gbe/evw303 Annotation Seabuckthorn (Hippophae rhamnoides) is a dioecious shrub commonly used in the pharmaceutical, cosmetic, and environmental industry as a source of oil, minerals and vitamins. In this study, we analyzed the transposable elements and satellites in its genome. We carried out Illumina DNA sequencing and reconstructed the main repetitive DNA sequences. For data analysis, we developed a new bioinformatics approach for advanced satellite DNA analysis and showed that about 25% of the genome consists of satellite DNA and about 24% is formed of transposable elements, dominated by Ty3/Gypsy and Ty1/Copia LTR retrotransposons. FISH mapping revealed X chromosome-accumulated, Y chromosome-specific or both sex chromosomes-accumulated satellites but most satellites were found on autosomes. Transposable elements were located mostly in the subtelomeres of all chromosomes. The 5S rDNA and 45S rDNA were localized on one autosomal locus each. Although we demonstrated the small size of the Y chromosome of the seabuckthorn and accumulated satellite DNA there, we were unable to estimate the age and extent of the Y chromosome degeneration. Analysis of dioecious relatives such as Shepherdia would shed more light on the evolution of these sex chromosomes. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2018
Number of the records: 1