Number of the records: 1
Microtubule-dependent targeting of the exocyst complex is necessary for xylem development in Arabidopsis
- 1.
SYSNO ASEP 0475903 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Microtubule-dependent targeting of the exocyst complex is necessary for xylem development in Arabidopsis Author(s) Vukašinović, Nemanja (UEB-Q) ORCID
Oda, Y. (JP)
Pejchar, Přemysl (UEB-Q) RID, ORCID
Synek, Lukáš (UEB-Q) RID, ORCID
Pečenková, Tamara (UEB-Q) RID, ORCID
Rawat, Anamika (UEB-Q)
Sekereš, Juraj (UEB-Q) RID, ORCID
Potocký, Martin (UEB-Q) RID, ORCID
Žárský, Viktor (UEB-Q) RID, ORCIDNumber of authors 9 Source Title New Phytologist - ISSN 0028-646X
Roč. 213, č. 3 (2017), s. 1052-1067Number of pages 16 s. Language eng - English Country US - United States Keywords secondary cell-wall ; tracheary element differentiation ; cortical microtubules ; plasma-membrane ; vesicle trafficking ; secretory pathways ; auxin transport ; exocytosis ; deposition ; thaliana ; conserved oligomeric Golgi (COG) complex ; exocyst ; microtubules ; secondary cell wall ; tracheary elements ; xylem Subject RIV EB - Genetics ; Molecular Biology OECD category Cell biology R&D Projects GA15-14886S GA ČR - Czech Science Foundation (CSF) Institutional support UEB-Q - RVO:61389030 UT WOS 000393875400012 DOI 10.1111/nph.14267 Annotation Cortical microtubules (MTs) play a major role in the patterning of secondary cell wall (SCW) thickenings in tracheary elements (TEs) by determining the sites of SCW deposition. The EXO70A1 subunit of the exocyst secretory vesicle tethering complex was implicated to be important for TE development via the MT interaction. We investigated the subcellular localization of several exocyst subunits in the xylem of Arabidopsis thaliana and analyzed the functional significance of exocyst-mediated trafficking in TE development. Live cell imaging of fluorescently tagged exocyst subunits in TE using confocal microscopy and protein-protein interaction assays were performed to describe the role of the exocyst and its partners in TE development. In TEs, exocyst subunits were localized to the sites of SCW deposition in an MT-dependent manner. We propose that the mechanism of exocyst targeting to MTs involves the direct interaction of exocyst subunits with the COG2 protein. We demonstrated the importance of a functional exocyst subunit EXO84b for normal TE development and showed that the deposition of SCW constituents is partially compromised, possibly as a result of the mislocalization of secondary cellulose synthase in exocyst mutants. We conclude that the exocyst complex is an important factor bridging the pattern defined by cortical MTs with localized secretion of the SCW in developing TEs. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2018
Number of the records: 1