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The in silico identification and characterization of a bread wheat/Triticum militinae introgression line

SYSNO ASEP	0475902
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	The in silico identification and characterization of a bread wheat/Triticum militinae introgression line
Author(s)	Abrouk, Michael (UEB-Q)_RID Balcárková, Barbora (UEB-Q) Šimková, Hana (UEB-Q)_{RID, ORCID} Komínková, Eva (UEB-Q)_{RID, ORCID} Martis, M.M. (DE) Jakobson, I. (EE) Timofejeva, L. (EE) Rey, Elodie (UEB-Q)_RID Vrána, Jan (UEB-Q)_{RID, ORCID} Kilian, A. (AU) Jarve, K. (EE) Doležel, Jaroslav (UEB-Q)_{RID, ORCID} Valárik, Miroslav (UEB-Q)_{RID, ORCID}
Number of authors	13
Source Title	Plant Biotechnology Journal. - : Wiley - ISSN 1467-7644 Roč. 15, č. 2 (2017), s. 249-256
Number of pages	8 s.
Language	eng - English
Country	US - United States
Keywords	crop improvement ; powdery mildew ; common wheat ; chromosomes ; genome ; resistance ; plant ; recombination ; evolution ; barley ; GenomeZipper ; alien introgression ; comparative analysis ; chromosome rearrangement ; chromosome translocation ; linkage drag
Subject RIV	EB - Genetics ; Molecular Biology
OECD category	Plant sciences, botany
R&D Projects	LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA14-07164S GA ČR - Czech Science Foundation (CSF)
Institutional support	UEB-Q - RVO:61389030
UT WOS	000394570600010
DOI	10.1111/pbi.12610
Annotation	The capacity of the bread wheat (Triticum aestivum) genome to tolerate introgression from related genomes can be exploited for wheat improvement. A resistance to powdery mildew expressed by a derivative of the cross-bread wheat cv. Tahti x T. militinae (Tm) is known to be due to the incorporation of a Tm segment into the long arm of chromosome 4A. Here, a newly developed in silico method termed rearrangement identification and characterization (RICh) has been applied to characterize the introgression. A virtual gene order, assembled using the GenomeZipper approach, was obtained for the native copy of chromosome 4A, it incorporated 570 4A DArTseq markers to produce a zipper comprising 2132 loci. A comparison between the native and introgressed forms of the 4AL chromosome arm showed that the introgressed region is located at the distal part of the arm. The Tm segment, derived from chromosome 7G, harbours 131 homoeologs of the 357 genes present on the corresponding region of Chinese Spring 4AL. The estimated number of Tm genes transferred along with the disease resistance gene was 169. Characterizing the introgression's position, gene content and internal gene order should not only facilitate gene isolation, but may also be informative with respect to chromatin structure and behaviour studies.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2018

Number of the records: 1