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Transcriptional regulation of male-sterility in 7B-1 male-sterile tomato mutant
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SYSNO ASEP 0475872 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Transcriptional regulation of male-sterility in 7B-1 male-sterile tomato mutant Author(s) Omidvar, Vahid (UEB-Q)
Mohorianu, I. (GB)
Dalmay, T. (GB)
Zheng, Y. (CN)
Fei, Z. (CH)
Pucci, A. (IT)
Mazzucato, E. (US)
Večeřová, R. (CZ)
Sedlářová, M. (CZ)
Fellner, Martin (UEB-Q) RID, ORCIDNumber of authors 10 Article number e0170715 Source Title PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 12, č. 2 (2017)Number of pages 19 s. Language eng - English Country US - United States Keywords tapetum-degeneration-retardation ; male reproductive development ; early anther development ; differential expression ; lycopersicon-esculentum ; stamenless-2 mutant ; abscisic-acid ; arabidopsis-thaliana ; brassica-napus ; cell-wall Subject RIV EB - Genetics ; Molecular Biology OECD category Plant sciences, botany R&D Projects LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000393712500013 DOI 10.1371/journal.pone.0170715 Annotation The 78-1 tomato (Solanum lycopersicum L. cv Rutgers) is a male-sterile mutant with enhanced tolerance to abiotic stress, which makes it a potential candidate for hybrid seed breeding and stress engineering. To underline the molecular mechanism regulating the male-sterility in 78-1, transcriptomic profiles of the 78-1 malesterile and wild type (WT) anthers were studied using mRNA sequencing (RNA-Seq). In total, 768 differentially expressed genes (DEGs) were identified, including 132 upregulated and 636 downregulated transcripts. Gene ontology (GO) enrichment analysis of DEGs suggested a general impact of the 78-1 mutation on metabolic processes, such as proteolysis and carbohydrate catabolic process. Sixteen candidates with key roles in regulation of anther development were subjected to further analysis using qRT-PCR and in situ hybridization. Cytological studies showed several defects associated with anther development in the 78-1 mutant, including unsynchronized anther maturation, dysfunctional meiosis, arrested microspores, defect in callose degradation and abnormal tapetum development. TUNEL assay showed a defect in programmed cell death (PCD) of tapetal cells in 78-1 anthers. The present study provides insights into the transcriptome of the 78-1 mutant. We identified several genes with altered expression level in 78-1 (including beta-1,3 glucanase, GA2oxs, cystatin, cysteine protease, pectinesterase, TA29, and actin) that could potentially regulate anther developmental processes, such as meiosis, tapetum development, and cell-wall formation/degradation. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2018
Number of the records: 1