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De novo transcriptome assembly of heavy metal tolerant Silene dioica
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SYSNO ASEP 0475870 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title De novo transcriptome assembly of heavy metal tolerant Silene dioica Author(s) Čegan, R. (CZ)
Hudzieczek, V. (CZ)
Hobza, Roman (UEB-Q) RID, ORCIDNumber of authors 3 Source Title GENOMICS DATA - ISSN 2213-5960
Roč. 11, MAR (2017), s. 118-119Number of pages 2 s. Language eng - English Country NL - Netherlands Keywords genome ; Silene dioica ; RNA-Seq ; Transcriptome ; Heavy metal tolerance ; Sex chromosomes Subject RIV EB - Genetics ; Molecular Biology OECD category Plant sciences, botany Institutional support UEB-Q - RVO:61389030 UT WOS 000395325800030 DOI 10.1016/j.gdata.2017.01.004 Annotation Silene dioica is a dioecious plant of the family Caryophyllaceae. In the present study, we used Illumina sequencing technology (MiSeq) to sequence, de novo assembly and annotate the transcriptomes of male and female copper tolerant S. dioica individuals. We sequenced the normalized mRNA of roots, shoots, flower buds and flowers for each sex. Raw reads of the transcriptome assembly project for S. dioica male and female individual have been deposited in NCBI's Sequence Read Archive (SRA) database with the accession number SRP094611. The Trinity and Detonate program was used to de novo assembly 92,347 transcripts for male and 94,757 transcripts for female transcriptome. The assembled transcriptome sequences for S. dioica male and female individuals can be accessed at NCBI with the following accession numbers: GFCG00000000 (male), GFCH00000000 (female). The obtained transcriptomic data will be useful for further studies focusing on copper tolerance, comparative transcriptome analysis with other Silene species and sex chromosomes evolution. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2018
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