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Myrica rubra leaves as a potential source of a dual 5-LOX/COX inhibitor

SYSNO ASEP	0474824
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Myrica rubra leaves as a potential source of a dual 5-LOX/COX inhibitor
Author(s)	Langhansová, Lenka (UEB-Q)_{RID, ORCID} Landa, Přemysl (UEB-Q)_{RID, ORCID} Kutil, Zsófia (UEB-Q) Tauchen, Jan (UEB-Q) Maršík, Petr (UEB-Q)_{RID, ORCID} Rezek, Jan (UEB-Q)_{RID, ORCID} Lou, J.D. (CN) Yun, Z.L. (CN) Vaněk, Tomáš (UEB-Q)_{RID, ORCID}
Number of authors	9
Source Title	Food and Agricultural Immunology - ISSN 0954-0105 Roč. 28, č. 2 (2017), s. 343-353
Number of pages	11 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	in-vitro ; antiinflammatory activity ; zucc. leaves ; 5-lipoxygenase ; sieb. ; cyclooxygenase ; constituents ; antioxidants ; myricitrin ; metabolism ; Myrica rubra ; anti-inflammatory ; cyclooxygenase 1 ; cyclooxygenase 2 ; 5-lipoxygenase
Subject RIV	FR - Pharmacology ; Medidal Chemistry
OECD category	Medicinal chemistry
R&D Projects	LH12165 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA16-07193S GA ČR - Czech Science Foundation (CSF)
Institutional support	UEB-Q - RVO:61389030
UT WOS	000394647700015
DOI	10.1080/09540105.2016.1272554
Annotation	Myrica rubra Sieb. et Zucc. is a valuable fruit tree that is used in Chinese, Japanese and Taiwanese traditional medicine. We investigated the anti-inflammatory activity of M. rubra leaves extracted with four different solvents. Total phenolics were determined using the Folin-Ciocalteu method. Extracts were investigated for their inhibitory activity toward the pro-inflammatory enzymes cyclooxygenase-1 and2 (COX-1, COX-2) and 5-lipoxygenase (5-LOX). The ethanol extract of M. rubra leaves demonstrated a strong inhibition of prostaglandin E-2 (PGE(2)) biosynthesis catalyzed by both COX-1 (93.42%) and COX-2 (75.71%) and leukotriene B-4 (LTB4) formation catalyzed by 5-LOX (82.72%). Further we identified selective COX-1 inhibition by the n-butanol and aqueous fractions of the ethanol extract (with an IC50 for COX-1 inhibition of 1.07 and 0.71 mu gmL(-1) (,) respectively) and dual 5-LOX/COX inhibition by the ethyl acetate fraction (with an IC50 of 3.29 for COX-1, 2.54 for COX-2 and 8.30 mu gmL(-1) for 5-LOX).
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2018

Number of the records: 1