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Rye B chromosomes encode a functional Argonaute-like protein with in vitro slicer activities similar to its A chromosome paralog
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SYSNO ASEP 0473677 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Rye B chromosomes encode a functional Argonaute-like protein with in vitro slicer activities similar to its A chromosome paralog Author(s) Ma, W. (DE)
Gabriel, T.S. (DE)
Martis, M.M. (DE)
Gursinsky, T. (DE)
Schubert, V. (DE)
Vrána, Jan (UEB-Q) RID, ORCID
Doležel, Jaroslav (UEB-Q) RID, ORCID
Grundlach, H. (DE)
Altschmied, L. (DE)
Scholz, U. (DE)
Himmelbach, A. (DE)
Behrens, S.E. (DE)
Banaei-Moghaddam, A.M. (DE)
Houben, A. (DE)Number of authors 14 Source Title New Phytologist - ISSN 0028-646X
Roč. 213, č. 2 (2017), s. 916-928Number of pages 13 s. Language eng - English Country GB - United Kingdom Keywords rna-polymerase-ii ; ribosomal-rna ; accessory chromosomes ; plant argonautes ; gene-expression ; evolution ; transcription ; pseudogenes ; sequences ; identification ; Argonaute ; B chromosomes ; B-located genes ; gene erosion. gene expression ; pseudogenization ; Secale cereale Subject RIV EB - Genetics ; Molecular Biology OECD category Plant sciences, botany R&D Projects LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GBP501/12/G090 GA ČR - Czech Science Foundation (CSF) Institutional support UEB-Q - RVO:61389030 UT WOS 000391452300039 DOI 10.1111/nph.14110 Annotation B chromosomes (Bs) are supernumerary, dispensable parts of the nuclear genome, which appear in many different species of eukaryote. So far, Bs have been considered to be genetically inert elements without any functional genes.
Our comparative transcriptome analysis and the detection of active RNA polymerase II (RNAPII) in the proximity of B chromatin demonstrate that the Bs of rye (Secale cereale) contribute to the transcriptome. In total, 1954 and 1218 B-derived transcripts with an open reading frame were expressed in generative and vegetative tissues, respectively. In addition to B-derived transposable element transcripts, a high percentage of short transcripts without detectable similarity to known proteins and gene fragments from A chromosomes (As) were found, suggesting an ongoing gene erosion process.
In vitro analysis of the A- and B-encoded AGO4B protein variants demonstrated that both possess RNA slicer activity. These data demonstrate unambiguously the presence of a functional AGO4B gene on Bs and that these Bs carry both functional protein coding genes and pseudogene copies.
Thus, B-encoded genes may provide an additional level of gene control and complexity in combination with their related A-located genes. Hence, physiological effects, associated with the presence of Bs, may partly be explained by the activity of B-located (pseudo) genes.Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2018
Number of the records: 1