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Flow Analysis and Sorting of Plant Chromosomes
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SYSNO ASEP 0471598 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Flow Analysis and Sorting of Plant Chromosomes Author(s) Vrána, Jan (UEB-Q) RID, ORCID
Cápal, Petr (UEB-Q) RID, ORCID
Šimková, Hana (UEB-Q) RID, ORCID
Karafiátová, Miroslava (UEB-Q) RID, ORCID
Čížková, Jana (UEB-Q) RID, ORCID
Doležel, Jaroslav (UEB-Q) RID, ORCIDSource Title Current protocols in cytometry - ISSN 1934-9300
Roč. 78, Oct 10 (2016), 5.3.1-5.3.43Number of pages 43 s. Language eng - English Country US - United States Keywords cell cycle synchronization ; chromosome genomics ; chromosome isolation Subject RIV EB - Genetics ; Molecular Biology R&D Projects LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 DOI 10.1002/cpcy.9 Annotation Analysis and sorting of plant chromosomes (plant flow cytogenetics) is a special application of flow cytometry in plant genomics and its success depends critically on sample quality. This unit describes the methodology in a stepwise manner, starting with the induction of cell cycle synchrony and accumulation of dividing cells in mitotic metaphase, and continues with the preparation of suspensions of intact mitotic chromosomes, flow analysis and sorting of chromosomes, and finally processing of the sorted chromosomes. Each step of the protocol is described in detail as some procedures have not been used widely. Supporting histograms are presented as well as hints on dealing with plant material; the utility of sorted chromosomes for plant genomics is also discussed. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2017
Number of the records: 1