Number of the records: 1

Flow Analysis and Sorting of Plant Chromosomes

SYSNO ASEP	0471598
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Ostatní články
Title	Flow Analysis and Sorting of Plant Chromosomes
Author(s)	Vrána, Jan (UEB-Q)_{RID, ORCID} Cápal, Petr (UEB-Q)_{RID, ORCID} Šimková, Hana (UEB-Q)_{RID, ORCID} Karafiátová, Miroslava (UEB-Q)_{RID, ORCID} Čížková, Jana (UEB-Q)_{RID, ORCID} Doležel, Jaroslav (UEB-Q)_{RID, ORCID}
Source Title	Current protocols in cytometry - ISSN 1934-9300 Roč. 78, Oct 10 (2016), 5.3.1-5.3.43
Number of pages	43 s.
Language	eng - English
Country	US - United States
Keywords	cell cycle synchronization ; chromosome genomics ; chromosome isolation
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	UEB-Q - RVO:61389030
DOI	10.1002/cpcy.9
Annotation	Analysis and sorting of plant chromosomes (plant flow cytogenetics) is a special application of flow cytometry in plant genomics and its success depends critically on sample quality. This unit describes the methodology in a stepwise manner, starting with the induction of cell cycle synchrony and accumulation of dividing cells in mitotic metaphase, and continues with the preparation of suspensions of intact mitotic chromosomes, flow analysis and sorting of chromosomes, and finally processing of the sorted chromosomes. Each step of the protocol is described in detail as some procedures have not been used widely. Supporting histograms are presented as well as hints on dealing with plant material; the utility of sorted chromosomes for plant genomics is also discussed.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2017

Number of the records: 1