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Chromosome-specific sequencing reveals an extensive dispensable genome component in wheat
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SYSNO ASEP 0471226 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Chromosome-specific sequencing reveals an extensive dispensable genome component in wheat Author(s) Liu, M. (CN)
Stiller, J. (AU)
Holušová, Kateřina (UEB-Q) ORCID
Vrána, Jan (UEB-Q) RID, ORCID
Liu, D. (CN)
Doležel, Jaroslav (UEB-Q) RID, ORCID
Liu, C. (AU)Number of authors 7 Article number 36398 Source Title Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 6, NOV 8 (2016)Number of pages 9 s. Language eng - English Country GB - United Kingdom Keywords triticum-aestivum l. ; fusarium crown rot ; pan-genome ; hexaploid wheat ; bread wheat ; draft genome ; rna-seq ; maize ; transcriptome Subject RIV EB - Genetics ; Molecular Biology R&D Projects LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GBP501/12/G090 GA ČR - Czech Science Foundation (CSF) Institutional support UEB-Q - RVO:61389030 UT WOS 000392054800001 DOI 10.1038/srep36398 Annotation The hexaploid wheat genotype Chinese Spring (CS) has been used worldwide as the reference base for wheat genetics and genomics, and significant resources have been used by the international community to generate a reference wheat genome based on this genotype. By sequencing flow-sorted 3B chromosome from a hexaploid wheat genotype CRNIL1A and comparing the obtained sequences with those available for CS, we detected that a large number of sequences in the former were missing in the latter. If the distribution of such sequences in the hexaploid wheat genome is random, CRNILA sequences missing in CS could be as much as 159.3 Mb even if only fragments of 50 bp or longer were considered. Analysing RNA sequences available in the public domains also revealed that dispensable genes are common in hexaploid wheat. Together with those extensive intra-and interchromosomal rearrangements in CS, the existence of such dispensable genes is another factor highlighting potential issues with the use of reference genomes in various studies. Strong deviation in distributions of these dispensable sequences among genotypes with different geographical origins provided the first evidence indicating that they could be associated with adaptation in wheat. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2017
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